食用植物油中动物源性成分PCR 检测方法的建立.pdf

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现代食品科技 Modern Food Science and Technology 2012, ,

食用植物油中动物源性成分 PCR 检测方法的建立

赵琳娜,王丹,胡凤月,吴鹏,韩月贝,杨红莲
(北京市食品安全监控中心,北京 100041)
摘要:本文建立了应用 PCR 技术快速鉴别食用植物油中动物源性成分的方法。以食用植物油中掺入的动物源性基因为靶标,自
行设计 16S rRNA 基因通用引物,同时选用动物物种特异性引物进行 PCR 扩增,分别得到相应的目的片段。通过三对 16S rRNA 基因
通用引物,建立了从食用植物油中快速检测是否含有动物源性成分的方法;利用所选的动物物种特异性引物,进一步建立了从食用植
物油中鉴定猪、牛、羊、鸡、鱼 5 种动物源性成分的方法,此方法能检测出含有 %(m/m)动物源性成分的植物油。
关键词:食用植物油;动物源性成分;掺入;PCR
文章篇号:1673-9078(2012)5-588-592
Detection of Animal-derived Ingredients
in Edible Vegetable Oils by PCR Method
ZHAO Lin-na, WANG Dan, HU Feng-yue, WU Peng, HAN Yue-bei, YANG Hong-lian
(Beijing Municipal Center for Food Safety Monitoring, Beijing 100041, China)
Abstract: In this article, a rapid and simple method of animal-derived ingredients in edible vegetable oils was established. Two pairs of
universal primers to animal species and five pairs of specific primers to pig, bovine, sheep, chicken and fish were designed for PCR
amplification. The amplicons of universal primers and species-specific primers were used for animal-derived ingredients detection. Sensitivity
and specificity of the newly developed method were confirmed with a detection level of % (m/m).
Key words: edible vegetable oils; animal-derived ingredients; adulteration; PCR

近年来,不法生产企业或人员将加工后的废弃油用劣质畜禽肉、动物下水等加工提炼的废弃油脂本身
脂作为食用油非法销售或在食品加工业中非法使用的就含有动物成分的特点,以食用