文档介绍：Construction of Plant Expression Vector and Transformtion for of
BmK ANEPⅢ Gene
HUANG Ting-ting,LAI Lin-lin,WANG Yi, ZHANG Jing-hai,SONG Yong-bo
(School of Biological Science and Biopharmacy, Shenyang Pharmaceutical University,Shenyang, 110016)
Abstract: CONCLUSION Constructed the plant expression vector harboring BmK ANEPⅢ(anti-neuroexcitation peptide from Buthus martensii Karsch) gene, obtained engineering strain BI121-DE35S-ANEPⅢ/ EHA105 for ic transformation. METHODS Using molecular cloning technique, inserted ANEPⅢ gene (added Kozak sequence to 5' end and the sequence of ER stranded peptide to 3' end) into vector pBin438, and further cloned sequences from pBin-ANEPⅢ which included CaMV DE35S promoter, Ω'enhancer and ANEPⅢ gene to pBI121-Gusint-D to get pBI121-DE35S-ANEPⅢ. The binant plasmid was transferred into EHA105 by freezing-thaw method, tri-parental mating method and electro transformation to obtained transformant. RESULTS essfully constructed plant binary expression vector pBin-ANEPⅢ and pBI121-DE35S-ANEPⅢ, obtained engineering strain pBI121-DE35S-ANEPⅢ/ EHA105. OBJECTIVE to improve our plant expression system to increase the expression level of the target protein.
Key words:BmK ANEPⅢ, vector construction, plant expression vector, transfection
BmK ANEPⅢ植物表达载体构建及根癌农杆菌转化条件考察
黄婷婷,来琳琳,王轶,张景海,宋永波
(沈阳药科大学生命科学与生物制药学院, 辽宁沈阳 11