文档介绍：毛细管电泳法测定麻黄及麻杏石甘汤中主成分的含量
景浩然,郭怀忠*,王子君,王敏,张斌
(河北大学药学院,河北保定 071002;
河北省药物质量研究重点实验室,河北保定 071002)
摘要:目的建立了毛细管区带电泳法测定麻黄及麻杏石甘汤中麻黄碱和伪麻黄碱含量的方法。方法电泳条件:电泳缓冲液为50 mmol/L硼砂-20 mmol/L苏氨酸缓冲溶液(1 mol/);紫外检测波长210 nm,分离电压15 kV;以苯巴比妥为内标物,采用压力进样(10 cm×20 s),柱温:室温。~213 μg/~84 μg/mL,, μg/ μg/mL, μg/ μg/mL。将该法用于麻黄中麻黄碱与伪麻黄碱的含量测定,%%。结论本法操作简便、快速、经济、灵敏度高,麻黄及麻杏石甘汤中麻黄碱及伪麻黄碱可获得满意的分离,可作为麻黄和麻杏石甘汤中麻黄碱和伪麻黄碱的含量测定方法。
关键词:高效毛细管电泳法麻黄麻杏石甘汤麻黄碱伪麻黄碱
Determination of ponent in Ephedra and Maxingshigan Decoction by Capillary Zone Electrophoresis
JING Haoran, GUO Haizhong*, WANG Zijun, WANG Min, ZHANG Bin
(School of Pharmacy, Hebei University, Baoding 071002;
Hebei Key Laboratory of Drug Quality Research)
Abstract: Objective To establish a method for the determination of ephedra ephedrine and pseudoephedrine in ephedra and maxingshigan decoction by capillary zone The conditions of the experiment were optimized with a fused-silica capillary of 60 cm×50 μm . (50 cm effective length ) in a running of 50 mmol/L borax-20 mmol/L threonine () with an applied voltage of 15 kV(room
第一作者介绍:景浩然,女,研究生,Email:haoranjing1980@
通讯联系人:郭怀忠,男,副教授,主要从事中药分析方面的研究,Tel:(0)**********,Email:ghuaizh@
temperature). Sample introduction was performed by hydrodynamic injections(10 cm
×20 s) an