文档介绍：β淀粉样蛋白对小胶质细胞合成一氧化氮的影响
作者:韩笑峰吕丽葛汝丽唐荣华
【摘要】目的探讨β淀粉样蛋白(amyloid betapeptide, Aβ)诱导小胶质细胞活化后κ轻链核因子(nuclear factorkappa B, NFκB)的表达及一氧化氮(NO)水平的变化。方法 Aβ干预纯化培养的小胶质细胞,观察其形态学变化,采用镉还原法测定NO水平,免疫细胞化学方法研究NFκB的表达。结果 500 nmol/L及1000 nmol/L Aβ干预组细胞形态呈“阿米巴样”,细胞核内NFκB的表达增加(P<),培养基中NO浓度升高(P<)。结论 Aβ激活小胶质细胞NFκB途径大量合成NO,可能参与阿尔茨海默病(Alzheimer’s disease, AD)的致病过程。
【关键词】β淀粉样蛋白小胶质细胞κ轻链核因子一氧化氮阿尔茨海默病
【Abstract】 Objective To study the morphological changes of microglial cell activated by amyloid betapeptide, the expression of nuclear factorkappa B increased in nucleus and the levels of nitric oxide in cultural medium. Methods Purified microglial cells were intervened by amyloid betapeptide and the morphological changes were observed . The cadmiumreduction method was used to determine the levels of nitric oxide in culture medium, immunocytochemical method to investigate changes of nuclear factor
kappa B. Results There were no statistical significant differences between the experimental group of 100 nmol/L amyloid betapeptide and the control group (P>). In the experimental groups of 500 nmol/L and 1000 nmol/L amyloid betapeptide, microglial cells were bigger, the expression of nuclear factorkappa B increased in nucleus and the levels of nitric oxide were higher in culture medium than control group (P<).Conclusion The amyloid betapeptide could participate in the processes