文档介绍：骨质疏松骨折愈合过程中TGF┐β1 mRNA的基因表达
【关键词】骨质疏松
关键词: 骨质疏松;骨折;转化生长因子β;核酸杂交
摘要:目的探讨Ⅰ型骨质疏松骨折愈合的病生机制,检测骨质疏松性骨折愈合过程中TGF-β1 mRNA的基因表达模式及细胞定位. 方法利用去势SD大鼠造成Ⅰ型骨质疏松及其骨折模型,通过原位杂交、斑点杂交检测骨痂局部TGF-β1 mRNA的表达. 结果骨质疏松骨折与正常骨折愈合过程中TGF-β1 mRNA基因表达的细胞定位未见差别;TGF-β1 mRNA表达的变化模式与正常骨折愈合过程相似. 结论 TGF-β1基因表达的改变与骨质疏松骨折愈合的病生改变未见关联.
Keywords:osteoporosis;fractures;transforming growth fac-tor beta;nucleic acid hybridization
Abstract:AIM To assess the changes of gene expression of the transform growth factors-β1 in the process of the healing of the osteoporotic fracture in ovariectomized female SD Ovariectomized female SD rats were used as model of type I osteoporosis and osteoporotic fracture and the changes of gene expression of the transforming growth fac-tors-β1 were detected in the process of the healing of the os-teoporotic fracture through in situ and blot There was no difference of the cellular localiza-tion in TGF-β1 expression between the processes of the nor-mal
and osteoporotic bone healing by in situ hybridization;The pattern of the gene expression of TGF-β1 mRNA was similar to the normal in the process of the osteoporotic bone healing by dot blot Expres-sion of TGF-β1 mRNA is not related to the pathophysiological changes in the process of osteoporotic bone healing.
0 引言
转化生长因子β(TGF-β)是骨组织中含量最丰富的细胞生长因子之一[1] ,它既具有促进成骨细