文档介绍：纳洛酮对大鼠心室肌细胞L-型钙通道的影响
王群超
【摘要】目的研究纳洛酮对正常大鼠心室肌细胞L型钙电流(ICa,L)的影响,探讨纳洛酮对心肌收缩力直接作用。方法采用急性酶解法获得大鼠的单个心室肌细胞,·ml-1和100μg·ml-±±±(n=11,P<)。I-V曲线随纳络酮浓度增加逐渐上移,但对激活电位和翻转电位无明显影响。20μg·ml-1和100μg·ml-±%±%(n=5,P<)。纳络酮对激活曲线并未产生显著影响。结论纳洛酮对大鼠心室肌细胞L型钙通道具有直接的抑制作用,其抑制程度具有浓度依赖性。
【关键词】纳洛酮;心室肌细胞;L-型钙电流;膜片钳技术
Effects of naloxone on L-type calcium channels in isolated cardiomyocytes of SD rat
Wang Qun- of anesthesiology,First Affiliated Hospital of Dalian Medical University,Dalian 116011, China.
【Abstract】 Objective To study the effects of naloxone on L-type calcium channel current in SD rat ventricular aim was to determine the non-opioid effect on direct cardiac muscle Single ventricular cell of SD rat was obtained by an enzymatic dissociation method,the whole-cell patch clamp recording technique was used to record the change of calcium current .Results Naloxone 20μg·ml-1 and 100μg·ml-1 decreased peak current of ICa,L from ± to ± and to ±(n=11,P<).The I-V curve of ICa,L was shifted upwards with higer concentration,but the activation voltage,and the reversal petential of ICa,L were not altered by 20μg·ml-1 and 100μg·ml-1 reduced the peak current of ICa,L by ±%