1244例慢性前列腺炎患者病原微生物检测及耐药性分析.doc

文档介绍：1244例慢性前列腺炎患者病原微生物检测及耐药性分析
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作者:徐亚丽王崇义葛宏兵
【摘要】目的探讨慢性前列腺炎患者前列腺中病原微生物及其耐药性。方法取前段尿(VB1)及前列腺液,进行常规外观、显微镜镜检、分离培养(细菌、真菌、支原体)及衣原体检测,采用API与ATB系统鉴定细菌;K-B纸片法测定细菌的耐药性;PCR法测定葡萄球菌的MceA 基因;表型确证试验检测大肠埃希菌与克雷伯菌的超广谱β-内酰胺酶(ESBL),Mycoplasma IST2试剂检测支原体及对9种抗生素的耐药性;采用chemtrueTMchlamydia 抗原检测法检测衣原体。结果 1244例前列腺液中702份标本中检出病原微生物786株,最常见的病原微生物为细菌356株(%)、支原体306株(%)和衣原体119株(%)。葡萄球菌是引起慢性细菌性前列腺炎的最主要的病原菌,203株中126株为耐甲氧西林葡萄球菌(MRS),其对除万古霉素外的其他12种抗生素均有较高的耐药率;306株支原体对9种抗生素的耐药率分别为氧氟沙星(%)、环丙沙星(%)、红霉素(%)、阿奇霉素(%)、克拉霉素(%)、四环素(%)、强力霉素(%)、交沙霉素(%)、原始霉素(%);31株大肠埃希菌和克雷伯菌中,ESBL阳性12株,%;38株肠球菌中,链霉素高度耐药17株,庆大霉素高度耐药15株,链霉素+庆大霉素均高度耐药12株,耐万古霉素肠环球菌(VRE)2株。结论慢性前列腺炎病因复杂,病原微生物种类较多,多重耐药菌感染常见,加强病原微生物及耐药性检测,对于慢性前列腺炎的诊断与治疗具有十分重要的意义。
【关键词】慢性前列腺炎病原菌耐药性葡萄球菌支原体衣原体
【Abstract】 Objective To study the pathogen and its drug-resistance in expressed prostatic secretion(EPS) of 1244 prostatitis patients. Methods The first-voded of urine(VB1) and EPS were collected for microscopic examine、fractional culture(bacteril,fungus,mycoplasma)and chlamdiae test, the bacterias were confirmed with API and ATB systerm its drug-resistance were examined by using K-B test; The Meca gene of us was examined by polymerase chain reaction(PCR);the extended spectrum beta-lactamase(ESBL) was detected by phenotypic confirmatory;nine drug-resistance of plasma were confir