文档介绍：C-erbB-2在贲门癌中的扩增及其临床意义
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作者:杨文杰王学智岳秀兰白晓荃
【摘要】目的:探讨贲门癌C-erbB-2癌基因扩增情况及其临床意义。方法:应用差别PCR技术检测32例贲门癌C-erbB-2癌基因扩增。结果:贲门腺癌C-erbB-2扩增率为50%;贲门癌C-erbB-2基因扩增与组织学分化程度、浸润程度均无关(P>),与淋巴结转移有关(P<)。结论:C-erbB-2癌基因扩增与贲门癌发生有密切关系,原癌基因C-erbB-2扩增可作为贲门癌淋巴结转移的生物学标志。
【关键词】贲门癌;C-erbB-2;差别PCR;癌基因扩增
Abstract Objective:To explore the amplication of C-erbB-2 oncogene in Gastric cardia adenocarcinoma(GCA) and its clinical :Oncogene C-erbB-2 amplification was examined by differential polymerase chain reaction in 32 cases of GCA. Results:16 out of 32 cases of GCA were found to have C-erbB-2 amplification. C-erbB-2 amplification in GCA was not correlated to histology differentiation and invasion degrees, but to lymph node metastasis. Conclusion: These results suggest that there is a close relationship between amplification of C-erbB-2 and GCA,and amplification of C-erbB-2 may be a biological marker for lymphatic matastasis in the patients with GCA.
Key words Gastric cardia adenocarcinoma;C-erbB-2;Differential polymerase chain reaction;Oncogene amplification
贲门癌(gastric cardia adenocarcinoma,GCA)是我国常见的恶性肿瘤之一,是指发生在胃贲门部,也就是食管胃交界线下约2cm范围内的腺癌。它是胃癌的特殊类型,但它又与其它部位的胃癌不同,具有自己的解剖组织学、病理学特性、临床表现、独特的诊断和治疗方法。GCA预后极差,中晚期患者5年生存率仅10%,侵袭和转移是GCA患者死亡的主要原因。现有研究表明,胃癌、结直肠癌、乳腺癌、卵巢癌、食管癌均伴有不同程度的C-erbB-2扩增,并与肿瘤的发生发展、转移、预后有关。贲门癌与C-erbB-2扩增关系的研究在国内外未见相关报道。为了明