文档介绍：DCSIGNR在人胎盘滋养层细胞中的表达及意义
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【摘要】目的: 探讨DCSIGNR在人胎盘绒毛组织中的定位及在体外培养滋养层细胞上的表达。方法: 建立稳定的滋养层细胞原代培养体系, 采用免疫组化单染及荧光双染的方法检测不同孕期正常胎盘绒毛组织及体外培养滋养层细胞上DCSIGNR的表达。结果: DCSIGNR主要表达于胎盘滋养层细胞、 Hofbauer细胞及胎盘微血管内皮细胞的胞质及包膜, 在原代培养的人绒毛膜滋养层细胞中的DCSIGNR的表达与在体组织表达一致。结论: DCSIGNR表达于不同孕期的胎盘组织及体外分离培养滋养层细胞, 为研究滋养层细胞上该受体在宫内感染中的作用提供体外实验的细胞学基础。
【关键词】滋养层细胞 DC SINGR 免疫组织化学宫内感染
[Abstract] AIM: To study the expression of DCSIGNR in human placenta and trophoblast cells cultured in vitro, and provided a basis for experiment in vitro aiming to investigate the mechanism of receptormediated intrauterine transmission. METHODS: Primary culture system of human chorionic trophoblast cells were established. The expression of DC
SIGN and DCSIGNR was determined by Immunohistochemistry(IHC) stain. RESULTS: DCSIGNR was mainly expressed in the membrane and plasm in placental trophoblast cells, Hofbauer cells and placental vascular endothelial cells. CONCLUSION: The expression of DCSIGNR in human placenta and trophoblast cells cultured in vitro is determined by IHC; The study provides a cellular basis for experiment in vitro aiming to investigate the mechanism of receptormediated intrauterine transmission.
[Keywords]trophoblast cells; DCSIGNR; IHC; intrauterine infection
病毒如何突破胎盘屏障的第一道防线滋养层细胞?其具体传播途径尚不明确。研究发现, 病毒可通过受体介导途径侵入细胞[1]。滋养层细胞表面存在有此作用的受体。从人胎盘库中筛选出一种未知的钙离子依赖C型凝