文档介绍：人早孕胎盘绒毛膜滋养层细胞的培养
作者:李富军,王雪萍,刘斌,李焰,李洁
【Abstract】AIM: To prepare highly purified human trophoblast cells from human placentae at the first trimester of normal pregnancy for further study on the function of human placentae villi. METHODS: A standard trypsinDNase dispersion method was used to isolate the trophoblast cells and cell purity was determined by immunostaining for markers of fibroblasts (antivimentin). The structural features were revealed by transmission electron microscopy. RESULTS: The cell viability evaluated by trypan blue exclusion was greater than 90%. And light microscopic examination revealed that the cells were epitheliallike cells, with patchy spreading. The cells were positive for cytokeratin and negative for vimentin, indicating the absence of contaminating elements. Transmission electron microscopy revealed that the cells had fine structural features typical of trophoblasts. CONCLUSION: The method, simple and easy to operate, can be used in studying the function of human placentae villi during pregnancy.
【Keywords】 trophoblast/cytology; cell culture; persons
【摘要】目的: 培养纯度较高的人绒毛膜滋养层细胞, : 胰蛋白酶DNase消化法培养人绒毛膜滋养层细胞,间接免疫染色进行细胞纯度检测,: 倒置显微镜下,可见细胞为上皮样细胞形态,,波型蛋白染色阴性,: 该法简便易行,可获得合乎试验要求的人绒毛膜滋养层细胞.
【关键词】滋养层/细胞学;细胞培养;人
0引言
人胎盘绒毛膜滋养层细胞对研究胎盘绒毛在妊娠期间的作用及其机制有很重要的生物学意义[1-8