文档介绍：MCP1基因A2518G多态性与不稳定性心绞痛关系的初步分析
(作者:___________单位: ___________邮编: ___________)
作者:师干伟,何,高磊,戚猛,沈丹丹,钱志宏,许联红
【摘要】目的: 探讨中国苏南地区汉族人群单核细胞趋化蛋白1(MCP1)基因启动子A2518G单核苷酸多态性与不稳定性心绞痛(UAP)的相关性。方法: 采用病例对照研究方法,选择临床确诊的UAP患者172例为病例组和经冠脉造影检查证实无冠脉病变者156例为对照组。利用聚合酶链式反应限制性酶切片段长度多态性技术(PCRRFLP)对MCP1基因 A2518G行多态性检测,并统计各基因型及等位基因的频率分布。结果: 与对照组相比,UAP组的AA,AG和GG基因型频率差异均无统计学意义(,)。%%,两组间差异无统计学意义(P=)。对多个相关因素行多元Logistic回归分析显示,MCP1基因A2518G单核苷酸多态性与UAP的发病无相关性()。结论: 在中国苏南地区汉族人群中,MCP1基因A2518G 单核苷酸多态性与UAP无显著相关性。
【关键词】不稳定性心绞痛; 单核细胞趋化蛋白
1; 单核苷酸多态性
[Abstract]Objective: To investigate the possible association between the monocyte chemoattractant protein1(MCP1) gene A2518G single nucleotide polymorphism(SNPs) in the promoter region with unstable angina pectoris(UAP) in Chinese Han population of Sunan region. Methods: This study was conducted with a casecontrol design including 172 patients with UAP(UAP group) and 156 control subjects who were free from coronary artery disease by Coronary Angiography checks(control group). Polymerase chain reactionrestriction fragment length polymorphism(PCRRFLP) was used for the detection of the A2518G polymorphism in MCP1 gene,and then the frequency of ype was statistically analyzed. Results: Compared with the control group, the frequencies of AA,AG and GG genotype were not significantly different in UAP group(P was , and , respectively). The prevalence of G allele was % and % respectively in the UAP group and control group; there was no difference between them in statistics(P=). Multivariate logistic regression analysis indicated that there was no significant correlation between MCP
1 gene A2518G polymorphism with UAP(). Conclusion: Our data shows that MCP1 gene A2518G polymorphism is not associated with the risk of UAP in the Chinese Han population of Suwan region.
[Key words]unstable angina pectoris; monocyte chemoattractant protein1; si