文档介绍：IL1B+3954/Taq Ⅰ基因多态性与慢性牙周炎易感性的关系
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作者:韩冰,苟建重,陈曦,李生斌
【摘要】目的探讨IL1B +3954/Taq Ⅰ基因多态性与慢性牙周炎易感性的关系。方法收集汉族人群116名受试者,其中包括66例中重度慢性牙周炎(CP)患者和50例牙周健康对照者,提取外周静脉血白细胞DNA,采用标准化的聚合酶链反应限制性片段长度多态性(PCRRFLP)的方法,进行IL1B+3954/Taq Ⅰ基因型的检测,比较各基因型检出率的差别。结果 IL1B+3954/Taq Ⅰ位点均以A1/A1基因型占优势, CP组A1/A2基因型检出率和健康对照组相比无统计学差异。结论 IL1B+3954/Taq Ⅰ等位基因2可能与汉族人群中重度慢性牙周炎遗传易感性无关。
【关键词】牙周炎;白细胞介素1;单核苷酸多态性;基因型;遗传易感性
ABSTRACT: Objective To study whether specific IL1B+3954/Taq Ⅰ genotype alleles are associated with chronic periodontitis (CP) in Chinese of the Han nationality. Methods CP group consisted of 66 patients while healthy controls group consisted of 50 subjects. Anti
coagulated peripheral blood sample was obtained from each subject and genomic DNA was extracted from each sample. Single nucleotide polymorphisms (SNPs) at IL1B+3954/Taq Ⅰ were analyzed by standard polymerase chain reactionrestriction fragment length polymorphisms (PCRRFLP) assay. Results In the control group, higher numerical values of the A1/A1 genotype were observed for the IL1B+3954/Taq Ⅰ SNPs (%), and the A2+ genotype was present in 16% of this sample. Statistical analysis revealed no significant difference between the two groups. Conlusion There is no evidence in our study supporting the association between single nucleotide polymorphisms (SNPs) of the IL1B+3954/Taq Ⅰ gene and