文档介绍：百草枯致小鼠帕金森病模型多巴胺能神经元损害作用的研究
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【摘要】目的研究环境毒素百草枯(PQ)诱发帕金森病(PD)过程中多巴胺能神经元的损害和基质金属蛋白酶(MMP)2、 MMP9的表达规律。方法 C57BL小鼠按体重10 mg/kg经腹腔注射PQ,分别观察动物行为,高效液相法测定纹状体部多巴胺(DA)含量的变化。免疫组化染色法观察和计数黑质(SN)酪氨酸羟化酶(TH)阳性神经元的数量,免疫组化法观察术后不同时间点MMP2、MMP9的动态变化。结果与对照组比较,模型组SN致密部TH阳性神经元数明显减少(P<),纹状体DA含量明显低于对照组(P<),MMP2、 MMP9的表达增强。结论大剂量的PQ可使小鼠产生类似于PD的行为和DA能神经元的损害,MMP2、 MMP9可能参与了DA能神经元的损害过程。
【关键词】百草枯;帕金森病;多巴胺能神经元
【Abstract】 Objective To investigate biological effect of paraquat (PQ), environmental factor, on dopaminergic neurons of substantia nigra (SN),and the role of matrix metalloproteinases (MMP) in the pathogenesis. Methods The C57BL mice were received intraperitoneal injections of 10 mg/kg PQ, and HPLC was us
ed to detect the content of dopamine (DA) in striatum. Immunohistochemistry assay was used to measure the immunoreactivity of tyrosine hydroxylase (TH) in SN. pared with that in control group, the amount of TH positive neuron in MPTP group was significantly decreased(P<), and the content of DA in striatum was decreased(P<), the expressions of MMP2 and MMP9 were significantly increased(P<) after treated with PQ might injury dopaminergic neurons of substantia nigra, MMP2 and MMP9 might attend the pathogenesis in C57BL mice.
【Key words】 Paraquat; Parkinson′s disease; Dopaminergic neurons
帕金森病(PD)确切病因尚不清楚。近年来