文档介绍：斑蝥酸钠诱导人食管癌Eca109细胞凋亡实验研究
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【摘要】目的观察斑蝥酸钠体外对人食管癌Eca109细胞凋亡的影响。方法应用MTT法,流式细胞仪检测,分析斑蝥酸钠对人食管癌Eca109细胞的生长和凋亡的影响。结果斑蝥酸钠作用24 h可使人食管癌Eca109细胞生长明显抑制(),并出现典型的凋亡峰。结论斑蝥酸钠对人食管癌Eca109细胞生长有抑制作用,可诱导食管癌细胞凋亡。
【关键词】斑蝥酸钠;食管癌;生长抑制;细胞凋亡;实验研究
Abstract:Objective To study sodium cantharidinate induced apoptosis of human esophageal carcinoma Eca109 in vitro. Methods The change of human esophageal carcinoma Eca109 treated with sodium cantharidinate were analyzed by MTT and FCM assay. Results Human esophageal carcinoma Eca109 were treated with sodium cantharidinate for 24 h, the cell proliferation was obviously inhibited () and the cells showed a typical apoptotic peak. Conclusions Sodium cantharidinate can inhibit cell proliferation and induce cell apoptosis of esophageal carcinoma cells Eca109.
Key words:Sodium cantharidinate;esophageal carcinoma;growth inhibition;apoptosis;Experimental Study
斑蝥酸钠(Sodium cantharidinate,SCA)是斑蝥素与氢氧化钠共热时水解生成[1]。临床单独使用或联合化疗对肝癌、胃癌、大肠癌等恶性肿瘤均有一定疗效,而且它还能配合放疗、化疗,提高治疗中晚期癌症的疗效,改善胸部不适、食欲减退、恶心呕吐等不同程度的放疗、化疗反应[2],具有较显著的抗癌抑癌和免疫调节的双重作用,是一种广谱抗癌新药。但目前对其抗癌机理研究的报道则甚少。笔者以人食管癌Eca109细胞系为对象,采用MTT、流式细胞仪检测食管癌细胞的生长和凋亡的变化,观察斑蝥酸钠对人食管癌Eca109细胞系生长和凋亡的影响,探讨其抗食管癌的作用机理,为临床应用提供理论依据。
1 实验材料
试药
RPMI1640培养液,美国GIBCO;新生牛血清,杭州四季青生物工程材料有限公司;胰酶,南京生兴生物技术有限公司;MTT,上海伯奥生物科技公司;二甲基亚砜(DMSO,分析纯),上海凌锋化学试剂有限公司,批号030820;顺铂(冻干型),齐鲁制药有限公司,批号04050111