文档介绍：水葫芦内生细菌脂肪酸生物标记特性研究收稿日期:2009-05-23 录用日期:2009-07-15
基金项目:福建省发改委项目(闽发高技[2005]1061号);省财政专项-福建省农业科学院科技创新团队建设基金(No. STIF Y03)
作者简介:蓝江林(1972─),副研究员,E-mail: lanfz2008@;*通讯作者(Corresponding author),E-mail: fzliubo@
蓝江林,刘波*,朱育菁,林营志,葛慈斌,刘芸
福建省农业科学院农业生物资源研究所,福州 350003
摘要:应用基于微生物细胞脂肪酸成分鉴定的全自动微生物鉴定系统,鉴定水葫芦内生细菌25株,分属15个属。共检测到27个脂肪酸生物标记(PLFAs),这些生物标记分为4种类型,即(1)高频次分布:在25株细菌中出现13~22次,属于细菌总体类群(general)的生物标记。(2)中频次分布:在25株细菌中出现5~7次,可以用于代表细菌属类群(genus)识别生物标记。(3)低频次分布:在细菌中的分布概率较小,可以用于指示特定细菌种间差异的生物标记。(4)微频次分布:仅在一种细菌种类出现,是细菌种(species)特征生物标记。利用脂肪酸生物标记分析同属细菌不同种的差异,可将微杆菌属分为2类,第1类包括菌株9Microbacterium barkeri和11Microbacterium imperiale,这两个菌株17:0 ISO和14:0 ISO的脂肪酸生物标记含量均为0。第2类包括10Microbacterium esteraromaticum、12Microbacterium lacticum和13Microbacterium liquefaciens,这3个菌株均含有17:0 ISO脂肪酸生物标记而区别于第一类菌株。利用脂肪酸生物标记的差异对25株内生细菌进行聚类分析,可将水葫芦内生细菌类群分为4类。不同植物内生菌群落中存在着特异的生物标记,利用特征脂肪酸生物标记的分析方法,分析水葫芦内生细菌的群体特性,对于植物内生菌微生物群落的研究具有重要意义。
关键词:内生细菌;脂肪酸生物标记;聚类分析
文章编号: 中图分类号:X522 文献标识码:A
Characteristics of PLFA Biomarkers for the Endophyte Bacteria inside Eichhornia crassipe (Mart.) Solms
LAN Jiang-lin, LIU Bo*, ZHU Yu-jing, LIN Ying-zhi, GE Ci-bin, LIU Yun
Agricultural Bioresource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003
Received 23 May 2009 accepted 15 July 2009
Abstract: The present paper dealt with isolation and identification of the endophyte bacteria from Eichhornia crassipe (Mart.) Solms. as well as characteristics of their PLFA biomarkers. Results showed that 25 species of endophyte bacteria were identified by means of microbe identification system (MIDI system from USA). There were 27 PLFA biomarkers detected in 25 species of endophyte bacteria, in which based on the distribution frequency in the bacteria 4 types were clustered . (1) high frequency related to characteristics of microbe in general, (2) middle frequency related to bacteria in general, (3) low frequency related bacterial genus, (4) tiny frequency related to species. According to PLFAs biomarkers, 5species