文档介绍：bcl┐2核酶对SMMC7721细胞端粒酶活性的影响作用
作者:张传山王文亮彭玮丹胡沛臻柴玉波张衍国
【关键词】肝肿瘤
关键词: 肝肿瘤;bcl-2;核酶;脱噬作用
摘要:目的观察bcl-2核酶对SMMC-7721细胞的作用,探讨bcl-2表达水平的改变对细胞端粒酶活性的影响. 方法经脂质体介导的方法将PMTr-neo(正向bcl-2核酶真核表达载体)导入SMMC7721细胞中,细胞克隆转移扩大培养后,采用TUNEL,TRAP-PCR-ELISA,免疫组化技术结合图像分析技术检测SMMC7721/PMTr-neo细胞凋亡、细胞端粒酶活性及P16,P21的改变. 结果较对照组SMMC7721/PMTr-neo细胞Bcl-2表达水平显著下降,伴有显著细胞凋亡现象;同时端粒酶活性下降,P16,P21表达水平显著增加. 结论 bcl-2核酶可促进SMMC7721细胞发生凋亡,并降低细胞端粒酶活性,提示Bcl-2表达水平的改变对细胞端粒酶活性有一定的影响.

Keywords:liver neoplasms;bcl-2;ribozyme;apoptosis

Abstract:AIM To observe the effect of bcl-2ribozyme on SMMC7721cells and to investigate bcl-2expression on cellu-lar apoptosis and telomerase PMTr-neo(sense bcl-2ribozyme eucaryotic expression
vector)was introduced into SMMC7221cells by using lipofectin media-tion,after cloning TUNEL and TRAP bination of ELISA and IHC bination of imaging analysis was used to determine SMMC7721/PMTr-neo apoptosis and telom-erase with control,SMMC7721/PMTr-neo presented a significant drop in bcl-2expression and an increase in P16and P21expression,-panied by a decline in remarkable apoptosis and telomerase bcl-2ribozyme promotes apoptosis of SMMC7721cells and reduces telomerase provide a theoretical basis for applying antisense technique to treatment of hepatocarcinoma.

0 引言

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