文档介绍：IGFBP-E2 增殖中的作用
作者:陈秋燕卢奕张健郭翔麦海强洪明晃
【摘要】【目的】探讨胰岛素样生长因子结合蛋白-6(IGFBP-6)在鼻咽癌患者病理组织中的表达与鼻咽癌患者临床特征与预后关系及其对鼻咽癌细胞株增殖的影响。【方法】采用免疫组化方法检测76例晚期鼻咽癌患者病理组织中IGFBP-6的表达,并对IGFBP-6的表达与鼻咽癌患者临床特征与预后关系进行探讨;用MTS方法检测IGFBP-E2增殖的影响。【结果】鼻咽癌IGFBP-6的表达阴性组和阳性组患者的5年总生存率分别为27%和73%(生存分析P = ),5年无局部区域复发生存率分别为38%和75%(P = ),5年无远处转移生存率分别为53%和89%(P = )。Cox模型多因素分析显示IGFBP-6的表达是76例鼻咽癌患者独立的预后影响因素(P = ),IGFBP-6阳性表达患者局部区域复发及远处转移风险降低。MTS检测结果显示IGFBP-E2的生长增殖(P < ),并且这种作用呈剂量依赖性。【结论】鼻咽癌组织中IGFBP-6的表达是晚期鼻咽癌患者的局部区域复发及远处转移风险有关的因素,其阳性表达提示患者局部区域复发及远处转移风险降低。体外MTS实验证明IGFBP-E2增殖。
【关键词】鼻咽癌; IGFBP-6; 预后; 增殖
Abstract: 【Objective】 To evaluate the expression of insulin-like growth factor binding prot
ein-6 (IGFBP-6) in nasopharyngeal carcinoma (NPC) patients and determine its clinical and prognostic significance. To study the effect of IGFBP-6 to NPC cell in the proliferation. 【Methods】 In this study, we evaluated immunohistochemical expression of IGFBP-6 in NPC tissue and determined its clinical and prognostic significance. The effect of IGFBP-6 to NPC cell E2 in proliferation were detected by MTS. 【Results】The 5-year overall survival (OS) rates of IGFBP-6 of negative group and positive group were 27% and 73% (survival analyses P = ). The 5-year loco regional relapse-free survival (LRFS) rates were 38% and 75% (P = ). The 5-year distant metastasis-free survival (DMFS) rates were 53% and 89% (P = ). Multivariate analysis in Cox model showed that IGFBP-6 were independent significant prognostic factor for 76 NPC patients. The positive expression of IGFBP-6 indicates a lower risk of developing loco regional relapse and distant metastasis. MTS showed that IGFBP-6 could decrease proliferation E2 (P < ) and this function was dose-dependent. 【Conclusion】 IGFBP-6 is an independent significant prognostic factor for loco regional 胰岛素样生长因子(insulin-like growth factor, IGF),是属于小分子多肽类生长因子,其氨基酸序列和胰岛素近似而得名。IGF的主要作用是调节碳水化合物、蛋白质和脂类的代谢,在肿瘤领域中,IGF与细胞增殖、分化和凋亡的关系日益受到关注。IGF系统由IGF-I和IGF-II及其受体和包