文档介绍：p38MAPK基因诱导胶质瘤细胞凋亡
作者:章必成李青巩西启叶菁陈广生王映梅林圣彩
【关键词】 p38分裂原激活蛋白激酶
关键词: p38分裂原激活蛋白激酶;基因转染;胶质瘤;凋亡;大鼠
摘要:目的研究p38MAPK基因转染大鼠胶质瘤细胞系C6后对其生物学特性的影响. 方法利用脂质体介导法将p38MAPK基因导入大鼠胶质瘤细胞系C6中,用免疫细胞化学染色检测其在细胞转染前后的表达情况,用HE染色、流式细胞仪等方法研究其对细胞形态、粘着状况和生长周期的影响. 结果转染pCMV5-p38MAPK质粒组p38MAPK蛋白表达阳性,细胞形态发生变化,贴壁性降低,出现大量凋亡细胞. 结论转染p38MAPK基因可诱导胶质瘤细胞凋亡.
Keywords:p38MAPK;gene transfection;glioma;apoptosis;rats
Abstract:AIM To study the effect of p38MAPK transfec-tion on the biological characteristics of glioma cell p38MAPK was transfected into glioma cell C6by of p38MAPK was detected by staining and flow cytometry were adopted to measure the cell morph
ology,adhesion and cell p38MAPK was expressed in transfected glioma cells,with cell biological characteristics changing and apoptotic cells Apoptosis of glioma cell could be induced by p38MAPK transfection.
0 引言
(mitogen-activated protein kinase,MAPK),已鉴定出4条,即细胞外信号调节蛋白激酶(extracellular signal-regulated protein kinase,ERK)通路,c-Jun氨基末端激酶(c-Jun N-terminal kinase,JNK)/应激活化蛋白激酶(stress-activated protein kinase,SAPK)通路,ERK5/大丝裂素活化蛋白激酶1(big MAP kinase,BMK1)、发育、分裂及细胞间的功能同步等多种生理过程,、炎症反应中具有重要作用外