文档介绍：放射抗拒性鼻咽癌细胞系的建立及差异表达基因
作者:王亚利,王西京,王中卫,金迎迎,李毅
【摘要】目的筛选同一来源放射敏感性不同鼻咽癌细胞基因差异表达,探讨鼻咽癌放射抗拒机理。方法 EE2R,采用BioStarHEE2R差异表达基因。EE2R细胞有差异表达的基因308条,上调176个,下调132个。在差异表达的基因中,有76个位点出现6倍以上的差异,其中36个下调、40个上调,包括与DNA修复相关、细胞周期、凋亡、细胞骨架相关蛋白、细胞增殖、代谢、蛋白质合成、信号转导、免疫相关等方面相关的基因,基因分布变化较明显的主要是DNA修复相关基因、细胞骨架、细胞周期和凋亡相关基因。E2细胞反复照射后产生放射抗拒性细胞,在基因水平上发生了某些突变,通过调控其中相关基因,就有可能调控细胞的放射敏感性。
【关键词】鼻咽癌;基因芯片;放射抗拒性
ABSTRACT: Objective To observe the differential gene expression in human nasopharyngeal carcinoma (NPC) cell line with different radiosensitivity by cDNA microarray analysis. Methods A radioresistant cell line, CNE2R, was established from a human nasopharyngeal carcinoma cells E2 by repeated Xray irradiation. The differential gene expression E
2 E2R was screened with cDNA microarray by BioStarH141s profile gene chip. Results There were expressed 308 genes to be screened out E2R cell line with different radiosensitivity and its E2 cell line, while 176 upregulated genes E2R cells and 132 downregulated genes were found. In them, there were 40 upregulated ones and 36 downregulated ones whose ratios were higher than or lower than . The different genes included DNA damage and repairrelated genes; cell cyclerelated and cytoskeletonrelated proteins; and apoptosisrelated genes. Conclusion The E2R cells were isolated from E2 cell line by repeated Xray irradiation. The stable radioresistance is the result of coeffect by polygene and multiple factors, which provide several gene targets to sensitize the radioresistant cells for improving the radiocurability of NPC.
KEY WORDS: nasopharyngeal carcinoma; cDNA microarray; radioresistance
放射治疗是鼻咽癌治疗最主要的手段,射线杀死了相对敏感的鼻咽癌细胞,而相对抵抗的亚细胞系则存活并增殖,成为复发的根源。近几年来,鼻咽癌的放射治疗的技术如调强放疗(intensity modulated radiation therapy, IMRT),图像引导的调强放疗(image guided radiation therapy, IGRT)等取得了巨大的进展,但是如何克服放射抵抗性尚无理想途径。E
E2R[1]。在此基础上对两株细胞进行2Gy照射后利用基因芯片分析二者差异表达的基因,以期发现与放射敏感性的相关基因,进一步建立鼻咽癌辐射相关基因芯片,为探讨鼻咽癌放射抗拒机理提供理论基础。
1 材料与方法
细胞培养
CNEE2R置于含100mL