文档介绍：枸杞多糖对荷瘤小鼠肿瘤微环境T淋巴细胞亚群及树突状细胞的影响
作者:何彦丽,应逸,许艳丽,苏俊芳,罗惠,王惠峰
【关键词】枸杞多糖
[摘要]目的:研究枸杞多糖(Lycium barbarum haride, LBP)对荷瘤小鼠体内肿瘤微环境中T淋巴细胞亚群和树突状细胞(dendritic cells, DCs)的影响,探讨LBP对荷瘤机体免疫逃逸的干预作用。方法:用LBP给H22荷瘤小鼠灌胃,连续2周后,测定肿瘤重量,采用流式细胞术检查肿瘤浸润淋巴细胞(tumorinfiltrating lymphocyte, TIL)中T淋巴细胞亚群和DCs的数量,以及协同刺激分子CD80(B71)表达的变化。结果:LBP可明显抑制肿瘤细胞的生长,增加肿瘤浸润CD4+、CD8+细胞的数量。LBP高剂量组CD4+和CD8+细胞数量的百分比高于模型组(P<)。LBP低剂量和高剂量组肿瘤浸润DCs数量及B71表达均较模型组升高,但差异无统计学意义。结论:LBP的抗肿瘤作用可能与恢复荷瘤小鼠TIL中CD4+、CD8+的细胞数量、解除机体的免疫抑制状态及增强机体的抗肿瘤免疫功能有关。LBP能否恢复荷瘤机体DCs的表型及功能尚待进一步研究。
[关键词]枸杞多糖; 免疫抑制; 淋巴细胞亚群; 肿瘤浸润淋巴细胞; 树突状细胞
Effects of Lycium barbarum haride on tumor microenvironment Tlymphocyte subsets and dendritic cells in H22bearing mice
ABSTRACT Objective: To study the effects of Lycium barbarum haride (LBP) on tumor microenvironment Tlymphocyte subsets and dendritic cells in H22bearing mice and the mechanisms for intervention of tumor immune escape by LBP. Methods: H22bearing mice were given LBP orally for two weeks. Tlymphocyte subsets and the phenotypes of dendritic cells in tumorinfiltrating lymphocytes (TIL) were detected by flow cytometry (FCM). Results: LBP could significantly increase the numbers of CD4+ and CD8+ T cells in TIL pared with those in model control group (P<). In model control group, the number of dendritic cells in tumor microenvironment decreased markedly, while in LBPtreated group, the increased number of dendritic cells and B71 expression were observed, but there were no significant differences between these two groups. Conclusion: LBP has antitumor effect probably by increasing the numbers of CD4+and CD8+ T cells in TIL to relieve the immunosuppression and enhance the antitumor function of the immune system. Bu
t whether LBP can recover the phenocyte and function of dendritic cells in H22bearing mice should be further studied.
KEY WORDS Lycium barbarum haride; immunosuppression; Tlymphocyte subsets; tumorinfiltrating lymphocyte; dendritic cells
近年来的研究发现:肿瘤患者体内存在多种免疫逃逸机制,表现为CD4