文档介绍：人参皂苷Rb1和Re对乌头碱所致心肌细胞损伤的保护作用
作者:董晞,赵世萍,刘岩,李平
【摘要】目的观察人参皂苷Rb1和Re对乌头碱导致的心肌细胞损伤的影响。方法选用乳鼠心肌细胞,观察人参皂苷Rb1和Re分别与乌头碱配伍使用后,乳鼠心肌细胞上清心肌酶谱的改变,采用RT-PCR的方法, mRNA的影响。结果人参皂苷Rb1和Re可以有效对抗由乌头碱导致的心肌酶谱改变。同时, mRNA表达上调。结论人参皂苷Rb1和Re与乌头碱配伍具有减毒增效的作用,这可能与人参皂苷减轻了乌头碱所致的心肌细胞损伤和改善钙离子通道异常表达有关。
【关键词】人参皂苷Rb1;人参皂苷Re;乌头碱;心肌酶谱;钙离子通道
Abstract:Objective To investigate the protective effect of ginsenoside Rb1 and Re on injury of the neonate rat cardiomyocyte induced by aconitine alkaloids. Methods The influence of aconitine and ginsenoside Rb1, Re together in neonate rat cardiomyocyte was observed respectively. The influence of ponents in neonate rat cardiomyocyte was examined by myocard zymogram. The expression of Ca2+ channel gene mRNA of neonate rat cardiomyocyte after treatment was observed by RT-PCR. Results The ginsenoside Rb1 and Re were able to decrease the release of AST and LDH, reverse mRNA abundance induced by aconitine. Conclusion The ginsenoside Rb1 and Re which together with aconitine can alleviate the side effects and boost up the therapeutic effects of aconitine. The action mechanism may be relation with that the ginsenoside Rb1 and Re can decrease injuries of the neonate rat cardiomyocytes and abundant expression of mRNA induced by aconitine.
Key words:aconitine alkaloids;ginsenoside Rb1;ginsenoside Re;myocard zymogram;Ca2+ channel
为了探讨人参与乌头碱的相互配伍作用,了解人参各成分与乌头碱之间是否有配伍减毒的作用,笔者采用了人参皂苷Rb1和Re分别与乌头碱共同作用于乳鼠心肌细胞,观察它们对于心肌细胞心肌酶谱变化是否存在相互作用,。
1 实验材料
动物
出生后1~3 d的Wistar大鼠,购于北京维通利华实验动物技术有限公司,动物合格证号:SCXK(京)2002-0002。
试剂和仪器
乌头碱(AC)、人参皂苷Rb1、人参皂苷Re(中国药品生物制品检定所产品,批号分别为110720-200410、110704- 200318、754-200115),DMEM高糖(GIBCO公司),胰蛋白酶(GIBCO公司),胎牛血清(北京市元亨圣玛生化制品有限公司),5-溴脱氧尿苷(Sigma公司),培养瓶(COSTOR公司),24孔培养板(NUNC公司)。SABC免疫细胞化学试剂盒、Mouse Anti-saconmericactin抗体、DAB染料(武汉博士德生物公司)。Trizol-100试剂盒(GIBICO,lnvitrogen Corporation生产),逆转录(RT)试剂盒、PCR试剂盒均为promega公司产品,购自北京华美转导科技有限公