文档介绍：人参皂苷Rg1对6-
作者:王明波陈文芳谢俊霞
【摘要】目的探讨人参皂苷Rg1对6-羟基多巴胺(6-OHDA) 。方法 ,观察人参皂苷Rg1预处理对6-OHDA毒性作用的影响,MTT法观察细胞存活率,实时荧光半定量反转录聚合酶链反应(real time RT-PCR)观察酪氨酸羟化酶(TH)和Bcl-2基因的表达情况。结果6-(F=,P<),人参皂苷Rg1预处理可对抗6-OHDA的毒性作用(F=,P<);6-OHDA可明显降低TH和Bcl-2基因的表达,人参皂苷Rg1预处理可明显逆转上述改变(F=、,P<)。结论人参皂苷Rg1可明显对抗6-,其作用机制可能与抗凋亡有关。
【关键词】人参皂甙类;羟基多巴胺类;神经元;细胞保护
[ABSTRACT] Objective To study the protective effect of ginsenoside Rg1 against 6-OHDA-induced neurotoxicity in cells. Methods cells were routinely cultured and pretreated with ginsenoside Rg1 to determine the protective effect of Rg1 against 6-OHDA-induced neurotoxicity. The cell viability was observed by MTT method. The gene expressions of TH and Bcl-2 were detected by real time RT-PCR method. Results 6-OHDA induced cell death in a dose-dependent manner. Pretreatment with ginsenoside Rg1 had neuroprotective effects on cell viability against 6-OHDA-induced toxicity. 6-OHDA decreased the gene expressions of TH and Bcl-2. These effects could be reversed by Rg1 pretreatment. Conclusion Ginsenoside Rg1 has neuroprotective effects against 6-
OHDA-induced neurotoxicity in cells, its mechansim may be related to its anti-apoptosis effects.
[KEY WORDS] Ginsenosides; Hydroxydopamines; Neurons; Cytoprotection