文档介绍：纳米金-表阿霉素复合体的体外抗肿瘤作用
赵晓旭潘运龙胡杨志覃莉丁晖巫青
【摘要】【目的】观察纳米金-表阿霉素复合体(EPI-AuNP)能否抑制人脐静脉内皮细胞(HUVEC)?人肝癌细胞(HepG2)的增殖?【方法】采用化学合成法制备EPI-AuNP,通过紫外-可见吸收光谱?荧光淬灭实验?动态光散射及Zeta电位变化对其进行鉴定?体外实验分为AuNP处理组?EPI处理组?EPI-AuNP处理组和空白对照组?将HUVEC?HepG2细胞分别接种于96孔板,培养24 h后各组分别加入AuNP溶液?EPI溶液?EPI-AuNP溶液和无血清培养液200 μL,继续培养24 h后:MTT比色法检测HUVEC?HepG2细胞生存率;紫外-可见分光光度法检测各细胞内EPI的积聚量?【结果】紫外-可见吸收光谱显示:AuNP的最大吸收峰在520 nm处,而EPI-AuNP在525 nm处?EPI (100 mg/L) ± ;EPI- ± ,P=?AuNP的平均粒径及Zeta电位分别为:( ± ) nm?(- ± )mV;EPI-AuNP为:( ± )nm?(- ± )mV,P<?体外实验:MTT比色法结果显示EPI处理组HUVEC?HepG2细胞的生存率分别为( ± )%?( ± )%;EPI-AuNP处理组:( ± )%?( ± )%,P=?【结论】成功合成EPI-AuNP复合体,体外实验证实其对HUVEC?HepG2细胞均具有增殖抑制作用?
【关键词】纳米金-表阿霉素复合体;HUVEC;HepG2细胞
Abstract: 【Objective】 To observe the in vitro antitumor effects of epirubicin-pounds (EPI-AuNP). 【Methods】 EPI-AuNP was prepared by chemosynthesis and investigated using UV-Vis spectrophotometer, fluorescence studie
s, dynamic light scattering,and zeta potential. Human umbilical vein endothelial cells (HUVEC) and HepG2 cells were divided into 4 groups: AuNP treatment group, EPI treatment group, EPI-AuNP treatment group and control group. After seeded in 96-well plate and cultured for 24 h separately, HUVEC and HepG2 cells were treated with 200 μL of AuNP,EPI,EPI-AuNP,and serum-free medium, respectively. Inhibition effect of each group on the HUVEC and HepG2 cells was assessed using MTT colorimetric method. UV-Vis spectrophotometer was applied to detect the cells epirubicin accumulation of different groups. 【Results】 A red shift in the SPR band maxima in the EPI-AuNP spectrum(λmax~525 nm) pared with the spectrum of AuNP alone (λmax~520 nm);The fluorescence intensity of EPI (100 mg /L) was ( ± ) and EPI-AuNP was ( ± ),P=. The hydrodynamic diameter of AuNP was ( ± ) nm while EPI-AuNP was ( ± ) nm. Meanwhile, the zeta potential of AuNP was (- ± ) mV while EPI-