文档介绍：发酵木糖生产乙醇菌株的筛选与鉴定
胡海军梁运祥葛向阳*
(华中农业大学农业部微生物学国家重点实验室发酵工程研究室,武汉 430070)
摘要:从花圃土和腐朽树皮中各筛选得到一株可以发酵木糖产生乙醇的菌株。/L。经过18S rDNA同源性的分析,菌株3鉴定为间型假丝酵母(Candida intermedia),而菌株621则为热带假丝酵母(Candida tropicalis)。对其发酵木糖、葡萄糖和混合糖的产物蒸馏后用气相色谱分析,主产物为乙醇,纯度高,副产物少。两菌株为下一步的菌种基因改造工作提供了研究基础。
关键词:木糖;乙醇;中型假丝酵母;热带假丝酵母
中图分类号: 文献标识码:文章编号:
Strains Collection for Produce Ethanol by Xylose-Fermening
Haijun Hu, Yunxiang Liang, Xiangyang Ge*
(Fermentation Engineering Research Laboratory, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, . China)
Abstract : Two strains of yeast respectively isolated from garden soil and rotten cortex could effectively produce ethanol from xylose in g/L and g/L yield. Through 18S rDNA sequence analysis, one strain is characterized as Candida intermedia and the other strain No. 621 shows highly identical with Candida tropicalis. Results of Gas Chromatography analysis to the distilled products fermented from xylose, glucose or mixture of them,showed these two strains have high efficiency production of ethanol in all of them,which could be used inal strains for further genic alteration work.
Keyword: Xylose; Ethanol; Candida intermedia; Candida tropicalis
前言
石油储备与消耗的现状,迫使人们寻找乙醇作为替代品。为了避免粮食与燃料之争,乙醇发酵的原料逐渐转向了以农林业废弃物为主的纤维质原料。这些农林业废弃物资源丰富,是变废为宝的可再生性原料。
纤维质原料主要由纤维素、半纤维素和木质素等组成。通过生物、物理化学等单一或联合的方