文档介绍：一起家庭聚集性诺瓦克样病毒感染的调查
作者:戴迎春,聂军,刘翼,李志峰,李建栋,俞守义
【关键词】人类杯状病毒
【Abstract】 AIM: To investigate the pathogeny, transmission route, clinical symptom and the pathogenesis of a family cluster nonbacterial diarrhea. METHODS: Stool specimens and suspicious food were detected by RTPCR and blasted with GenBank. RESULTS: The Norwalklike virus (NLVs) was detected from the stool specimens of the child, father, grandfather and the specimen of crab, and the homologous was 100%. CONCLUSION: The family cluster nonbacterial diarrhea is confirmed as a family cluster of NLVs infection. NLVs are detected in food, which is rarely reported in our country.
【Keywords】 human caliciviruses; Norwalklike virus; family cluster nonbacterial diarrhea
【摘要】目的: 调查一起家庭聚集性非菌性腹泻病原、传播途径、临床表现以及病原学特性. 方法: 感染者腹泻粪便及其可疑食物采用RTPCR方法检测,并测序. 结果: 患儿、患儿父亲、患儿外公粪便标本及蟹肉标本均扩增出NLVs目的片断,测序分析表明该四份标本同源性为100%. 结论: 证实该起家庭聚集性非菌性腹泻为诺瓦克样病毒感染所致,并在食物中检测到诺瓦克样病毒,初步确定了其传播途径.
【关键词】人类杯状病毒;诺瓦克样病毒;家庭聚集性非菌性腹泻
0引言
诺瓦克样病毒(Norwalklike virus, NLVs)是引起***和儿童非菌性急性胃肠炎的重要病原体,与札幌样病毒(Sapporolike virus, SLVs)合称人类杯状病毒(Human caliciviruses, HuCVs)[1]. 1972年,Kapikian首先用免疫电镜(IEM)在1968年美国Norwalk地区的一所学校暴发的胃肠炎患者粪便中检出了直径为27~32 nm的球状病毒颗粒,定名为诺瓦克病毒(Norwalk virus, NV) [2]. 本研究我们对在广州市进行HuCVs感染情况研究时[3]发现的一起家庭聚集性感染进行了病原学和流行病学研究,证明为NLVs所引起的家庭内病毒性腹泻暴发.
1对象和方法
首发腹泻病例: 女童, a, 于20031001晚10时发病,主要症状为恶心、呕吐(5~6次),并伴有腹痛、腹泻(2次),次日上午去医院就诊. 肌注灭吐灵10 mg,并口服先锋霉素Ⅵ胶