文档介绍：氧化苦参碱诱导骨肉瘤细胞凋亡的实验研究
【摘要】目的探讨不同浓度的氧化苦参碱对人骨肉瘤细胞的生长抑制和诱导凋亡作用。方法将不同浓度的氧化苦参碱作用于培养的OS732 骨肉瘤细胞, 通过MTT 比色法、流式细胞仪和DNA 凝胶电泳、光学显微镜技术观察检测细胞凋亡, 研究分析其对骨肉瘤细胞的生长抑制和诱导凋亡作用。,, %,%,%;%,%,%;DNA 电泳见DNA“梯形”图谱;显微镜显示细胞的凋亡形态。结论氧化苦参碱能显著抑制OS732 细胞增殖、促进其凋亡。
【关键词】骨肉瘤细胞氧化苦参碱凋亡
Abstract:ObjectiveTo study the growth-inhibitory effect and the mechanism on apoptosis induced by oxymatrine on OS732 a cells were treated with oxymatrine for 3 days. The cyto- toxicity was observed by MTT assay. The apoptotic rates were shown by flow cytometry (FCM ). Electron microscopy and DNA gel electrophoresis were applied to demonstrate of the presence of apoptosis. ResultsWhen a cells were treated with oxymatrine (, and mmol/L ) , the cytotoxicity indexes were % , % and % respectively, the apoptostic rates were %,%,%. The obvious apotosis morphological change
s in a cells were shown by electron microscope. ConclusionOxymatrine is able to inhibit the proliferation and induce the apoptosis of OS732 cells significantly. It is a potential and valuable proposal for clinical treatment of a.
Key words:a; Oxymatrine; Apoptosis
氧化苦参碱(oxymatrine)是从豆科植物苦参、苦豆子中提取的有效成分,具有广泛的生物活性,其抗病毒、抗炎及对中枢神经系统的镇静、镇痛、解热、降温作用和强心、降压、抗心率失常、调节免疫功能已被广泛用于临床各科[1]。近几年,氧化苦参碱的抑瘤作用已逐步被证实。如氧化苦参碱诱导人乳腺癌细胞的凋亡[2],对小鼠肉瘤S180的抑制