文档介绍：:~.—,章春桃,韦强,鲍国连,肖琛闻,季权安(浙江省农科院畜牧兽医研究所,浙江杭州310021)摘要:为建立兔波氏杆菌(Bb)的快速检测方法,本研究采用纯化重组兔BbfimN蛋白作为包被抗原建立了问接ELISA抗体检测方法。通过优化反应条件,。检测结果显示,该方法对兔Bb标准阳性血清检测的敏感性为l:2;而与兔巴氏杆菌、兔出血症病毒阳性血清无交叉反应。重复性试验表明,其组内和组间变异系数均小于10%。采用建立的间接ELISA方法对浙江省5家兔场采集的150份兔血清样品的检测阳性率为55%。该方法的建立为进一步研发兔BbELISA诊断试剂盒提供了实验依据。关键词:兔波氏杆菌;fimN蛋白;ELISA中图分类号:$:A文章编号:(2013)11-0899—binantfimNaSCOatlnqantiqenLILTYan,ZHANGChun-tao,WEIQiang,BAOGuo—lian,XIAOChen—wen,JIQuan—an(AnimalHusbandryandVeterinaryInstitute,ZhejiangAcademyofAculturalSciences,Hangzhou310021,China)Abstract:ToestablisharapiddetectionprotocolfortheantibodydetectionofBordetellabronchisepticainrabbits,,thecut—:—assayandintra—assayvariationwerelessthan10%.Inaddition,atotalof150rabbitserumsampleswhichcollectedfromfivedifferentfarmsofZhejiangprovinceweretestedbytheestablishedmethod,theseropositiveratewas55%.TherfimN—basedELISAprovidedatechnologicalbasisforthedevelopmen