文档介绍:脐带血清在骨髓造血祖细胞培养中的效应         摘要目的:探讨人脐带血清(CBS)在骨髓造血祖细胞培养中的效应。方法:用人骨髓细胞进行CFU-GM、CFU-E、BFU-E、CFU-GEMM培养。结果:CBS能直接刺激骨髓细胞CFU-GM的形成。与血型相同否无关。四人份以上的混合CBS(MCBS),刺激活性高且稳定。10%-CSF、、、-GM、CFU-E、BFU-E、CFU-GEMM所起的作用。MCBS可提高CFU-GM、CFU-E、BFU-E、CFU-GEMM常规体系的集落产率。在CFU-GM培养时,20%MCBS与100μg/LGM-CSF和20%马血清具有相同的效果。MCBS可直接刺激CFU-E形成。如培养体系内无BpA存在时,形成BFU-E、CFU-GEMM较少或几乎为零,当含BpA时,可形成一定量的BFU-E、CFU-GEMM集落。结论:CBS(MCBS)内:①含有GM-CSF、Epo等物质或因素;②具有刺激CFU-GM、CFU-E、BFU-E、CFU-GEMM的生长活性;③同血型或不同血型4人份以上混合,刺激活性高且稳定。具有良好的应用前景。关键词: 脐带血清; 骨髓; 造血祖细胞中分类号: -33文献标识码:A 文章编号:1000-6834(2000)01-0068-04EFFECTSOFCORDBLOODSERUMONCOLONYCULTUREOFBONEMARROWHEMATOPOIETICPROGENITORCELLSHUANGYou-zhang,LUOHui,YANGPing-di,SHENJian-liang(DepartmentofHematology,GeneralHospitalofNavy,Beijing10003)ABSTRACT Aim:ToprovideexperimentalproofsforCordBloodclinicalappliance,theeffectsofhumanCBS(CordBloodSerum):ObservedthecolonyformingcapacityofCFU-GM,CFU-E,BFU-E,CFU-GEMMwhentheywerecultivatedusinghBMNCwithCBS,MixedCordBloodSerum(MCBS)and/:10%CBScouldpromotetheformingofCFU-GMofBMCdirectly,(MCBS),nomatterwhetherthe“ABO”bloodgroupsbetweenthemweresameornot,theycouldproducemoreCFU-GMthansinglecopyortwo-copies-mixedCBSwhenused,-GM、CFU-E、BFU-E、CFU-GEMMinroutineculturesystem,and10%-CSF,,,-GM,CFU-E,BFU-E,CFU--GM,20%MCBShasthesameeffectas100μg/LGM-CSFwith20%%MCBScouldalsodirectlypromotetheformingofCFU--EandCFU-GEMMwithoutBpAandsomeBFU-EandCFU-:①SCBS(MCBS)containssuchsubstancesasGM-CSF,EPO,andsoon.②ThereexistssomeactivitieswhichcanpromotetheformingofCFU-GM,CFU-E,BFU-E,CFU-GEMMinCBS(MCBS).③Theseactivitieswouldbestrongerandmorestableiffourormo