文档介绍：(一/一)/Patched(+/一)双基因敲除鼠的构建辛世杰李璇MollyDuman—ScheelWeiDu【摘要】目的构建CycD(一/一)/Ptc(+/一)双基因敲除鼠以验证Hh传导系统与Rb系统的关系。方法应用已有的杂合子CycD基因敲除鼠CycD(+/一)与杂合子Ptc基因敲除鼠Ptc(+/一)进行交配繁殖,得到双基因杂合子敲除鼠CycD(+/一)/Ptc(+/一),再利用该双基因杂合子敲除鼠进行二次交配繁殖,得到实验组CycD(一/一)/Ptc(+/一)双基因敲除鼠。结果实验得到了双基因敲除鼠CycD(一/一)/Ptc(+/一)、双基因杂合子基因敲除鼠CycD(+/一)/Pte(+/一)、CycD单基因敲除鼠CycD(一/一)/Ptc(+/+)。结论通过本实验方法得到CycD(一/一)/Ptc(+/一)双基因敲除鼠,可用于进一步揭示Ptc基因与CycD基因相互影响调控的作用及Hedgehog与Rb信号传导通路在哺乳动物中的相关性。【关键词】CyclinD;Patched;基因敲除鼠ConstructionofCyclinD一,一/Patched(+/一doubleknockoutmicex|NShi-jie‘,LIXuan,MollyDuman—Scheel,Du.’DepartmentofVascularSurgery,FirstHospitalofChinaMedicalUniversity,Shenyang110001,ChinaCorrespondingauthor:XINShi-jic,Email:******@.ca【Abstract】0bjectiveToconstructCyclinD(CycD)(一/一)/Patched(Ptc)(+/一)~maleheterozygoteCycDknockoutmiceCycD(+/一)andmaleheterozygotePtcknockoutmicePtc(+/一),thusCycD(+/一)/Ptc(+/一)(一/一)/Ptc(+/一)(一/一)/late(+/一)doubleknockoutmice,CycD(+/一)/late(+/一)heterozygotedoubleknockoutmice,andCycDknockoutmiceCycD(一/一)/Ptc(+/+)(一/一)/Ptc(+/一)doubleknockoutmicethatcanbeusedtodis