DCN基因导入对自发性高血压大鼠肾脏纤维化的影响.doc

文档介绍：
DCN 基因导入对自发性高血压大鼠肾脏纤
维化的影响#
严雯1,冯敏2,汪道文3*
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(1. 华中科技大学同济医学院附属同济医院老年科,武汉 430030;
2. 华中科技大学同济医学院附属协和医院神经外科,武汉 430030;
3. 华中科技大学同济医学院附属同济医院,武汉 430030)
摘要:目的研究人核心蛋白聚糖(DCN)基因导入对自发性高血压大鼠(spontaneously
hypertensive rats, SHR)肾脏纤维化的逆转作用。方法 16 周龄雄性 SHR 经尾静脉注入重组腺
相关病毒(binant adeno-associated virus, rAAV)介导的人 DCN 病毒。16 周后处死动物测
定人 DCN 基因在各脏器中的表达及肾脏纤维化程度。结果经尾静脉注入重组腺相关病毒
介导的人 DCN 基因后,高血压大鼠的肾脏纤维化程度较对照组明显改善。结论人 DCN 基
因的导入可预防和逆转高血压所致的肾脏纤维化,为临床应用 DCN 基因治疗合并有肾脏损
伤的高血压提供了可能性。
关键词:高血压;肾脏纤维化;人核心蛋白聚糖基因
中图分类号:
The effects of human decorin gene on renal fibrosis in
spontaneously hypertensive rats
YAN Wen1, FENG Min2, WANG Daowen3
(1. Department of Geriatrics, Tongji Hospital, Tongji Medical College of Huazhong University of
Science and Technology, WuHan 430030;
2. Department of Neurosurgery, Union Hospital, Tongji Medical College of Huazhong University
of Science and Technology, WuHan 430000;
3. Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology,
WuHan 430030)
Abstract: Objective To investigate the reversal effects of DCN gene on SHR’s renal fibrosis.
Methods 16 week male SHRs were allocated randomly into three groups: saline (1ml), a single
dose of rAAV-GFP or rAAV-DCN (about 1×1011 virion particles per rat in 1 ml of saline solution)
via the tail vein into SHRs were performed respectively. At the end, we m