文档介绍：
超声波处理对蟑螂肠道放线菌分离效果的
影响
杨万赋1,王栋2*
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(1. 昆明卫生职业学院医技系,昆明 650600;
2. 红河学院生命科学与技术学院生物科学系,云南蒙自 661100)
摘要:为分离更多样的放线菌,而采用超声波预处理及稀释平皿分离测数法研究了超声波预
处理对蟑螂肠道放线菌分离效果的影响。结果表明:超声波处理能显著增加可培养放线菌数
量并缩短培养时间,随着超声波处理时间的增加,蟑螂肠道中分离的放线菌总数呈先增后减
的趋势。处理 3~21 分钟,高氏一号培养基上放线菌总数分别较对照提高 9%~%;处
理 24~30 分钟,高氏一号培养基上放线菌总数分别较对照降低 %~%。
关键词:微生物;放线菌;蟑螂肠道;超声波预处理;分离
中图分类号:
Intestinal Actinomyces Separation with Ultrasonic
Treatment
Yang Wanfu1, Wang Dong2
(1. Kunming health professional, KunMing 650600;
2. Hong He University, YunNan MengZi 661100)
Abstract: The purpose of this thesis is to optimizate the pretreatment method of actinomyces
which were difficultly isolated by the traditional pretreatment methods. The effect of ultrasonic
pretreatment on the isolation of cockroach's intestinal canal was studied by petri dish dilution and
isolation counting method. The findings of this study indicate that the ultrasonic pretreatment can
remarkably increase the number of educable actinomyces and shorten the cultivation time. In
addition, as the more time that ultrasonic pretreatment takes, the number of actinomyces isolated
from cockroach’s intestinal canal will firstly increase and then decrease. Specifically, the number
of actinomyces increase respectively 9%-% and %-% compared with the original
one when the ultrasonic pretreatment ta