敲低Txnip对高糖诱导的肾小管细胞凋亡的影响.doc

文档介绍：
敲低 Txnip 对高糖诱导的肾小管细胞凋亡的
影响#
韦金英,侯延娟,史永红**
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(河北医科大学病理教研室,石家庄 050017)
摘要:目的观察敲低 Txnip 对高糖诱导人肾小管上皮细胞凋亡的影响。方法将体外培养人
肾小管上皮细胞分为正常糖组、高糖组、高糖+质粒载体对照组及高糖+shRNA 组。采用原
位末端转移酶标记技术(TUNEL)及流式细胞术检测细胞凋亡;流式细胞术检测细胞 ROS
水平;Western blot 检测 caspase-3、cleaved caspase-3、Bax、Bcl-2、p38 MAPK、P-p38MAPK
及细胞色素 c 的表达。结果与正常糖组相比,高糖组肾小管上皮细胞 ROS 产生和细胞凋亡
明显增加,cleaved caspase-3 和 p-p38 MAPK 表达增高,Bax/Bcl-2 比率明显升高以及细胞色
素 c 易位。敲低 Txnip能够显著抑制高糖诱导的肾小管上皮细胞凋亡和 ROS 产生,下调 cleaved
caspase-3、和 p-p38 MAPK 的表达,减少 Bax/Bcl-2 比率和细胞色素 c 易位。结论敲低 Txnip
能够抑制高糖诱导的肾小管上皮细胞凋亡可能是通过减少 ROS 产生,保护线粒体功能,抑
制 p38MAPK 信号通路激活而实现的。
关键词:糖尿病肾病;氧化应激;人肾小管上皮细胞;凋亡;线粒体
中图分类号:R-332
Influence of Txnip interference on HG-induced human
kidney proximal tubular cell line apoptosis
WEI Jinying, HOU Yanjuan, SHI Yonghong
(Dept of Pathology, Hebei Medical University, Shijiazhuang 050017)
Abstract: To investigate the effect of Txnip interference on high glucose (HG)-induced apoptosis
in human kidney proximal tubular cell line (HK-2). Methods Cultured HK-2 were divided into
normal glucose group(NG),high glucose group (HG), HG+contol plasmid vector(HG+C) and
GH+ VDUP1 shRNA Plasmid (h) (HG+shRNA).Apoptosis of HK-2 was analyzed by DeadEnd™
Fluorometric TUNEL System and flow cytometry. ROS production was observed by flow
cytometry. The expression levels of Txnip, caspase-3, cleaved caspase-3, Bax, Bcl-2, p38 MA