文档介绍：
GDF-15 在曲古菌素 A 作用胃癌细胞
SGC-7901 后及胃腺癌组织中表达分析#
李云龙,崔武,李晓林,王浩,田瑜,曹志刚,佟柏峰,邹小明*
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(哈尔滨医科大学第二临床医学院,哈尔滨 150080)
摘要:目的: 研究 GDF-15 基因在 TSA 作用胃癌细胞系 SGC-7901 后及胃腺癌组织中的表达。
方法: 采用 Real-time PCR 检测胃癌细胞系 SGC-7901 经过 TSA 干预后 GDF-15 基因表达的
变化,免疫组化检测 GDF-15 基因在胃癌及癌旁正常组织中的表达。结果: GDF-15 基因在胃
癌细胞系 SGC-7901 经 75ng/ml TSA 作用 48 小时后,表达下调,差异具有显著性(P<),
免疫组化显示胃腺癌组织中 GDF-15 基因表达明显高于周围正常胃组织(P<)。结论:
GDF-15 基因表达下降可能在 TSA 促胃癌细胞系 SGC-7901 凋亡中发挥作用。GDF-15 基因
在胃腺癌组织中表达增高。
关键词:胃癌;GDF-15;TSA; SGC-7901
中图分类号:R4
Expression analysis of GDF-15 in the gastric cancer
SGC-7901 cells induced by TSA and in gastric
adenocarcinoma tissues
LI Yunlong, CUI Wu, LI Xiaolin, WANG Hao, TIAN Yu, CAO Zhigang, TONG
Baifeng, ZOU Xiaoming
(Second clinical medical college,Harbin medical university,Harbin 150080)
Abstract: In this paper,we study the expression of GDF - 15 gene in the gastric cancer cell line
SGC-7901 induced by TSA and in gastric carcinoma tissues. The Real - time PCR detection of
GDF - 15 gene expression changes in gastric cancer cell line SGC - 7901 after the TSA
intervention, immunohistochemical detection of GDF-15 gene expression in gastric cancer and
normal tissue adjacent to carcinoma .GDF - 15 gene in gastric cancer cell line SGC - 7901 through
75 ng/ml after 48 hours by TSA, expression decreased, with significant difference (P < ),
immunohistoche