枯草芽孢杆菌改善猪链球菌对Caco-2 细胞的破坏作用.doc

文档介绍：
枯草芽孢杆菌改善猪链球菌对 Caco-2 细胞
的破坏作用#
王志胜,杨倩,庾庆华**
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(南京农业大学动物医学院,南京,210095)
摘要:为探讨枯草芽孢杆菌拮抗猪链球菌感染的研究,本试验以 Caco-2 细胞为细胞模型,
通过活菌计数的方法研究了枯草芽孢杆菌拮抗猪链球菌黏附 Caco-2 细胞的情况;通过
transwell 细胞模型检测了枯草芽孢杆菌拮抗猪链球菌对 Caco-2 细胞通透性以及炎症细胞因
子 IL-8 的分泌的作用。结果显示,枯草芽孢杆菌能够明显减少猪链球菌对 Caco-2 细胞的黏
附,减弱猪链球菌对细胞通透性的破坏,并且减少炎症细胞因子 IL-8 的分泌。由此可见,
枯草芽孢杆菌能明显改善猪链球菌对 Caco-2 细胞的破坏作用,为枯草芽孢杆菌作为益生菌
的应用提供理论基础和试验依据。
关键词:枯草芽孢杆菌;链球菌;肠上皮细胞
中图分类号:
The Improving effect of Bacillus subtilis on the damage to
Caco-2 cells caused by us suis
Wang Zhisheng, Yang Qian, Yu Qinghua
(Veterinary college, Nanjing Agricultural University)
Abstract: In order to explore the antagonistic effect of Bacillus subtilis to us suis
infection, the antagonistic experiment was performed on Caco-2 cells to check the number of
us suis adhesion by bacteria count method. We also detected the paracellular
permeability and the IL-8 secretion after us suis infection or treated by Bacillus
subtilis on Caco-2 cells. The results showed that Bacillus subtilis could reduced the number of
us suis adhesion to Caco-2 cells and the inflammatory cytokines IL-8 expression
significantly. Thus, Bacillus subtilis could significantly improve the damaging effects of
us suis to Caco-2 cells, which provide a theoretical basis and experimental evidence
for using Bacillus subtilis as a probiotic applications.
Key words: Bacil