甜瓜果实八氢番茄红素脱氢酶基因(CmPDS)的克隆与表达分析.doc

文档介绍：
甜瓜果实八氢番茄红素脱氢酶基因(CmPDS)
的克隆与表达分析#
赵军林,于喜艳,王秀峰**
5
10
15
(山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,农业部黄淮地区园艺作
物生物学与种质创制重点实验室,山东泰安 271018)
摘要:以甜瓜自交系‘M01-3’果实 cDNA 为模板,采用 RT-PCR 和 RACE 技术,克隆了甜
瓜果实八氢番茄红素脱氢酶(PDS)基因 cDNA 全长序列,命名为 CmPDS(基因注册号:
KC507802)。序列分析表明,该基因有开放阅读框 1731bp,编码 576 个氨基酸,与其他植
物 PDS 氨基酸序列具有较高的同源性,尤其与黄瓜、南瓜、苦瓜 PDS 氨基酸序列高达 %
~ %。荧光定量分析表明,该基因在甜瓜不同器官中均有表达,在叶片和授粉后 25d 的
果实中表达量较高,在根中表达量最低;随果实发育成熟,该基因表达量呈现先升高后降低
趋势。
关键词:甜瓜;PDS 基因;克隆;表达
中图分类号:中图分类号:S652
文献标志码:A
Cloning and Expression Analysis of Phytoene Desaturase
Gene (CmPDS) from Fruit of Muskmelon
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Zhao junlin, Yu xiyan, Wang xiufeng
(College of Horticulture Science and Engineering, Shandong Agricultural University, State Key
Laboratory of Crop Biology, Key Laboratory of Biology and ic Improvement of
Horticultural Crops(Huanghuai Region), Ministry of Agriculture, P. R. China, Tai’ an, Shandong
271018, China)
Abstract: A full-length cDNA encoding Phytoene desaturase(PDS), named as CmPDS (GenBank:
KC507802), was isolated from the fruit of ‘M01-3’of Muskmelon(Cucumis melo L.)by RT-PCR
and RACE. The cDNA has an open reading frame of 1731bp and encodes a polypeptide of 576
amino acids. Sequence analysis showed that deduced CmPDS protein was highly homologous to
other PDS proteins from different species, especially with Cucurbita moschata, Momordica
charantia and munis, that the iden