白藜芦醇抗呼吸道合胞病毒作用体外实验研究.doc

文档介绍：
白藜芦醇抗呼吸道合胞病毒作用体外实验
研究#
谢秀春,徐建亚,汪受传**
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(南京中医药大学第一临床医学院,南京 210029)
摘要:目的:通过体外实验探讨白藜芦醇抗呼吸道合胞病毒(RSV)作用。方法:将 RSV
接种在 Hep-2 上,采用细胞病变抑制法(CEP)和 MTT 法,以利巴韦林作为阳性对照药物,
观察白藜芦醇在治疗、预防、直接抑制三种作用模式下细胞存活率。结果:三种作用模式
均有一定程度的抑制作用,且治疗作用和预防作用较明显,在预防作用模式下,白藜芦醇
组 CPE 与利巴韦林组相比明显减少;治疗作用模式下,与 RSV 组相比(P<),白藜芦醇
组对病毒的抑制率较高。结论:白藜芦醇对 RSV 具有一定的体外抑制作用。
关键词:白藜芦醇;呼吸道合胞病毒;体外
中图分类号:
Study on the Effect of Resveratrol against RSV in vitro
XIE Xiuchun, XU Jianya, WANG Shouchun
(The First Clinical College,Nanjing University of Chinese Medicine, NanJing 210029)
Abstract: Objective: To investigate the effect of resveratrol against respiratory syncytial virus
(RSV) through experiments of in vitro .Methods: The cell survival rate of resveratrol in the
treatment, prevention, direct inhibition of three kinds of mode were measured by cytopathic effect
reduction assay and MTT assay,when Hep-2 cell was infected with RSV and the antiviral effect of
resveratrol with ribavirin as the positive control : The three of action have a certain
degree of inhibition and the therapeutic effect and preventive effect is more pronounced. In
preventive effect mode,the CPE were typically reduced in resveratrol pared with
ribavirin group;In therapeutic effect mode,the virus inhibition rate of resveratrol group is higher
compared with RSV infected group(P <).Conclusion