文档介绍：基因组学与应用生物学,2013年,第32卷,第4期,第557-561页Genomics and Applied Biology, 2013, , , 557-561技术改进Upgraded TechnologyUPLC-MS/MS法同时测定益气养血口服液中的5种有效成分岑卫健1*范晓苏2朱平川1覃莎2王锦21广西大学亚热带农业生物资源保护与利用国家重点实验室,南宁, 530004; 2广西大学化学化工学院,南宁, 530004*通讯作者,weijianc@摘要建立了同时测定益气养血口服液中的淫羊藿苷、黄芪甲苷、黄芪皂苷域、五味子醇甲和麦冬皂苷D5种有效成分含量的超高效液相色谱-串联质谱(UPLC-MS/MS)分析方法,用Agilent Zorbax RRHDEclipse PlusC18(, )色谱柱,流动相:A相为乙腈溶液,%甲酸的水溶液, mL/min。在电喷雾电离(ESI)正离子模式下,采用的是多重反应监测模式(MRM)进行检测。结果表明,黄芪甲苷、黄芪皂苷域、淫羊藿苷、~12 mg/L、~12 mg/L、~18 mg/L、~ mg/L、~ mg/L;检出限分别为1滋g/L、1滋g/L、3滋g/L、2滋g/L、1滋g/L;%~104%;%。该方法简便、准确、快速、高灵敏度,已成功地用于实际的样品分析。关键词益气养血口服液,有效成分,超高效液相色谱-串联质谱(UPLC-MS/MS)Simultaneous Determination of Five ponents in YiqiyangxueOral Liquid by Ultra Performance Liquid Chromatography with TandemMass SpectrometryCen Weijian1*Fan Xiaosu2Zhu Pingchuan1Qin Sha2Wang Jin21 State Key Laboratory of Conseruation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, 530004; 2 School of Chem-istry and Chemical Engineering, Guangxi University, Nanning, 530004* Corresponding author, ******@DOI: .000557AbstractA method for the simultaneous determination of icariin, astragaloside郁, astragaloside域, schisandrinand ophiopogonin D in Yiqiyangxue oral liquid was established by ultra performance liquid chromatography wit