6个鸭茅品种（系）SSR遗传多样性分析.doc

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6 个鸭茅品种(系)SSR 遗传多样性分析#
蒋林峰,张新全,黄琳凯,谢文刚,闫艳红,刘伟,程良**
(四川农业大学动物科技学院草业科学系,四川雅安 625014)
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摘要:本研究以 6 个鸭茅品种(系)为供试材料,从 SSR 分子标记方面对其进行了种群内
及种群间遗传多样性和亲缘关系分析。结果表明:1)6 个鸭茅品种(系)间的平均遗传距
离为 ,低于 ,%的遗传变异存在于种质内部,种质间变异为 %;2)利
用 SSR 分子标记对 6 个鸭茅品种种质共 120 个单株进行分析,15 对引物共扩增出 114 条清
晰可辨条带,平均每对引物扩增的条带为 条,多态性比率为 100%,多态性信息含量为
~,平均为 。研究证明 SSR 标记用于鸭茅遗传多样性分析是可行的,在亲缘关系
鉴定上也具有一定参考性。
关键词:鸭茅;SSR;遗传多样性;亲缘关系
中图分类号:S543
15
ic Diversity in 6 Orchardgrass (Dactylis glomerata L.)
Cultivars (lines) Based on SSR Markers
JIANG Linfeng, ZHANG Xinquan, HUANG Linkai, XIE Wengang, YAN Yanhong,
LIU Wei, CHENG Liang
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(Department of grassland science, Animal Science and Technology College, Sichuan Agricultural
University, Ya’an, Sichuan, 625014, China)
Abstract: SSR markers were employed to investigate the ic diversity among and within 6
orchardgrass cultivars (lines). The results indicated: 1) The average number of ic distance
among the 6 cultivars (lines) of D. glomerata was , which was below , and % of
the ic diversity exist within the cultivar (lines), while ic difference among cultivars
(lines) was %. 2) 114 bands were amplified, on average bands were amplified by each
pair of primer. The rate of polymorphic sites was 100%, PIC ranged from to with an
average of . These results suggested that SSR mar