文档介绍：
Fine mapping and candidate gene analysis of gl-1, a gene
controlling trichome formation in rice#
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ZENG Ruizhen, LIU Ziqiang**
(College of Agriculture, South China Agricultural University)
Abstract: Leaf trichome plays an important role in protecting plants against insect herbivores, loss of
water through transpiration and UV irradiation. In our present study, SSSL-W24, a single segment
substitution line (SSSL) containing only one chromosome segment of the glabrous japonica cultivar
Star 99 in the ic background of the pubescent indica cultivar HJX74 showed glabrous
leaves and hulls. ic analysis of the F2 generation, derived from a cross between SSSL-W24 and
HJX74, showed that these traits are controlled by the recessive gene gl-1, which was mapped to the
short arm of chromosome 5. Fine mapping and high-resolution linkage analysis using 1585 F3 plants
and markers flanking gl-1 were carried out, and the gene was localized to a kb region that contains
seven annotated genes according to the genome sequence of japonica Nipponbare. Positional cloning of
this gene will assist selection of hybrid rice, facilitate the mechanization of agriculture and increase the
warehousing capacity of rice.
Key words: Crop ic breeding; Rice; Trichome; Single segment substitution line; Fine mapping;
Glabrous gene;
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0 Introduction
Aerial surfaces of most land plants have epidermal hairs called trichome. They are believed to
protect plants against insects, microbes, herbivores, and abiotic damages and to assist seed
dispersal[1,2]. Furthermore, seed trichomes of Gossypium plants, namely cotton fibers, are the most
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widely used natural fibers in the textile industry[3].
Glabrous mutants or cultivars are known in various plant species, including Arabidopsis[4],
rice[5], and maize[6], but few detailed studies on glabrousness were carried out except in
Arabidopsis.
Trichome patterning in Arabidopsis is controlled by several transcription f