文档介绍：
血管紧张素 II 对小鼠原代成骨细胞线粒体
功能影响#
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(1. 四川大学华西口腔医院修复科,成都 610041;
2. 四川大学口腔疾病研究国家重点实验室,成都 610041)
摘要:目的:研究血管紧张素 II 对小鼠成骨细胞线粒体功能的影响。方法:体外培养小鼠
原代成骨细胞,用 10-6M Ang II 刺激成骨细胞 24 小时,利用线粒体荧光探针测量线粒体
活性氧的产生,并检测线粒体氧化呼吸链酶活性,线粒体膜电位以及 ATP 的合成,确定 Ang
II 对小鼠成骨细胞线粒体功能活性的影响。结果:在经过 10-6M Ang II 刺激后,小鼠成骨
细胞线粒体内活性氧生成明显增加,氧化呼吸链酶活性受到抑制,线粒体细胞膜电位降低,
ATP 合成量下降。结论:Ang II 对小鼠成骨细胞线粒体功能活性有着明显的抑制作用,为
进一步研究高血压与骨吸收疾病相关性机制提供了新思路。
关键词:血管紧张素 II;成骨细胞;线粒体;活性氧;氧化呼吸链酶复合体
中图分类号:Q28
The effect of Angiotensin IIon the mitochondrial functions in
mice primary osteoblast culture
Wang Min1,2, LI Guangyue1,2, Yue Yuan1,2, Bai Congjia1,2, Zhang Ddongjiao1,2
(1. ,West China Hospital of Stomatology, Sichuan University,ChengDu
610041;
2. State Key Laboratory of oral disease, Sichuan University, ChengDu 610041)
Abstract: Objective :To analyze the effects of Angiotensin(Ang) Ⅱon mitochondrial functions
in primary mouse osteoblast culture. Methods: The mouse osteoblasts were cultured in vitro, and
treated with or without 10-6M AngⅡfor 24 hours. The generation of reactive oxygen species
(ROS) was detected by fluorescent probe MitoSox. The enzymatic activities of mitochondrial
respiratory plex, mitochondrial membrane potentials and ATP generations were also
evaluated to determine the effects of Ang II treatments on the mitochondrial fu