文档介绍：神经病理性疼痛大鼠行为学及生长相关蛋白表达的变化
厉彩霞林海王均炉△
温州医学院附属第一医院麻醉科
[摘要] 目的:观察大鼠坐骨神经结扎后神经病理性疼痛行为学以及生长相关蛋白-43(GAP-43)表达的变化。方法:I)模型,75只SD大鼠,随机分为三组(n=25)。对照组(con组):不接受任何手术操作;假手术组(sham组):只分离坐骨神经,I组:结扎坐骨神经。于术前2d,术后1、3、5、7、10、14、21、28d测定各组大鼠热缩足反射潜伏期(TWL),机械缩足反应阈值(MWT),并于术后3、7、14、21、28天各组随机抽取5只大鼠杀死,采用免疫组织化学方法测定脊髓背根节GAP-43表达的变化。结果:I组术后各日TWL,MWT明显降低(P<<),sham组和con组行为学无显著差异(P>)。CCI组较其它组GAP-43表达增高,I组中术后第三天表达开始增高,第七至十四天达最高峰,以后逐渐下降。I造模成功后大鼠痛觉及机械痛觉均过敏,且GAP-43表达增高。
[关键词] 神经病理性疼痛行为学改变 GAP-43
Behaviorl observation and changes of the expression of growth associated protein-43 in dorsal root ganglion of rats following ligaion of sciatic nerve
Li cai-xia, Lin-hai, Wang jun-lu Department of Anesthesiology,First Affiliated Hospital,Wenzhou Medical College,Wenzhou 325000,China
[Abstract] Objective To investigate the behaviorl observation and changes of growth associated protein-43 in dorsal root ganglion of rats with ligaion of sciatic Seventy-five SD rats were randomly divided into 3 groups :Control group(Con),Sham operation group (Sham),Chronic constriction injury I).CCI model was adopted ,thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) of rats were measured on1,2 pre-operative and 1,3,5,7,10,14,21,28 post-operative rats were randomly picked from each group on 3,7,14,21,28 post-operative days and the immunochemistry was adopted to detect the cha