文档介绍：摘要辣椒素是辣椒的主要功效成分。众多研究报告表明,辣椒素具有镇痛消炎、抑制癌症、调节血脂、消脂减肥等功能性作用,是一种具有广阔发展前景的生物碱。但其自身特有的强烈刺激性大大局限了辣椒素在医疗、美容等领域的广泛应用,而微胶囊化技术可以起到屏蔽物料味道和气味,减弱甚至消除辣椒素刺激性的作用。本课题以明胶和羧***纤维素(CMC)为壁材,安全无毒的谷氨酰***转氨酶(TG)为固化交联剂,采用复合凝聚法制备辣椒素微胶囊。研究了壁材的带电荷情况,辣椒素定量方法,微胶囊制备工艺,产品理化性质及其热稳定性。对明胶与CMC溶液在不同pH下的带电荷情况进行了考察,,当其溶液pH小于等电点时明胶离子带正电荷,大于等电点时明胶离子带负电荷,而CMC在实验范围内均带负电荷;对辣椒素的两种定量方法一紫外分光光度法和高效液相色谱法进行了比较,结果表明以上两种辣椒素的测定方法均符合本课题的实验要求,考虑到其应用广泛性选择了紫外分光光度法作为衡量辣椒素微胶囊产品包埋产率及效率的表征方法。以包埋产率及效率为考察指标,研究了壁材浓度、明胶与CMC添加量比例、芯材与壁材添加量比例、凝聚过程中溶液pH对制备工艺的影响。在单因素实验的基础上,采用正交试验对微胶囊制备工艺条件进行优化,得到的最佳工艺条件为:壁材浓度为1%,明胶与CMC比例为9:l,芯壁比为l:l,,%,%。对辣椒素及微胶囊的可接受度进行综合评分,,大大提高了其可接受性;对微胶囊产品的理化性质进行了考察。实验表明:%,,%;微胶囊产品粒径在100om左右,外形较为完整,呈不规则球形结构;傅立叶红外光谱检测表明微胶囊产品包埋效果良好;热重实验结果表明包埋后囊膜对辣椒素起到了一定的保护作用,提高了辣椒素的热稳定性。关键词:辣椒素:微胶囊;复合凝聚法;;谷氨酰***转氨酶 AB STRACT Capsaicin isthespicy and activeingredient hasmany functions,such asanalgesis,bacteriostasis,,regulating theblood fat, losingweight,antifatigue, itsstrong pungency limitstheapplication in all provides severaladvantages byimproving thephysical properties ofcore,shielding tasteand smell,controling releaseandsustaining release plex coacervation occurs under mild conditions and simple operation,whose products shows greatthermostability and thispaper,capsaicin Wasmicroencapsulated plex coacervation ofgelatin andCMC,nontoxic transglutaminase(TG)is thecross—linking harges ofgelatin andCMC,quantitative method ofcapsaicin,preparation processing,physical and chemicalproperties,and storage stability werestudied. The charges ofCMC andgelatin brought indifferentpH weretested,and the resultsshowed thatisoelectricpoint(IEP)of gelatin which usedinexperiment , thegelatin showspositive chargeunder IEP,and negativecharge above IEP,Oll the otherhand,the CMC always puts out negative ultraviolet