文档介绍：inogenes为例。,inogenes,在Gene下查找,图1进入下面的界面,图2以FSU_2293为例,基因注释为木聚糖酶,点击进去,图3发现FSU_2293,属于GH10,有CBM6,将此基因进行统计,见下表。GH10FSU_2293CBM6BTDxylanasexylanase 若是NCBI上没有显示相关的GH家族或CBM,则可以进行BLAST。点击图3中的“FASTA”,复制相关基因进行BLAST。例如CHU_0868,是糖基转移酶相关的基因,但是没有显示具体的GT家族。图4进行BLAST,图5点击相关的结构域,进入另一个网页,图6可以看到,此基因属于GT1家族。或是进行Pfam结构域搜索:网址:,输入相关基因,点击,图7可以看到,此基因具有GT1结构域,属于GT1家族。分别统计glycosidehydrolase(GH)、glycosyltransferase(GT)、 haridelyase(PL)、carbohydrateEsterase(CE)。对于glycosidehydrolase可以统计与纤维素降解和半纤维素降解相关的基因。格式见下表。注意:表中标红的列为必填项目,“CharacterizedActivity”为图2中的“Descriprition”。CAZyFamilyMYPLocusCBMFamilyInSilicoPredictionCharacterizedActivityGH(74)GHMYP_0567hypotheticalproteinGHMYP_0581hypotheticalproteinGHMYP_0913hypotheticalproteinGHMYP_1026hypotheticalproteinGHMYP_1096hypotheticalproteinGHMYP_3811endoglucanaseendoglucanaseGHMYP_4017xyloglucanasexyloglucanaseGHMYP_4345CBM9beta-xylosidase/endoglucanasebeta-xylosidase/endoglucanaseGHMYP_4348CBM4_9glycerolkinaseglycerolkinaseGH1MYP_2015beta-galactosidasebeta-galactosidaseGH2MYP_3616beta-mannosidasebeta-mannosidaseGH3MYP_0984b-glucosidaseb-glucosidaseGH3MYP_1602hypotheticalproteinGH3MYP_1909b-glucosidaseb-glucosidaseGH3MYP_2588xylosidase/arabinosidasexylosidase/arabinosidaseGH3MYP_3067glycosidehydrolaseGH3MYP_4245xylosidase/arabinosidasexylosidase/arabinosidaseGH3MYP_4961b-N-acetylglucosaminidaseGH5MYP_0458endoglucanaseEndoglucanaseGH5MYP_2390endoglucanaseendoglucanaseGH5MYP_1753beta-glycosidasebeta-glycosidaseGH5MYP_2157endo-1,4-beta-mannosidaseendo-1,4-beta-mannosidaseGH5MYP_2462beta-glycosidasebeta-glycosidaseGH5MYP_2666endoglucanaseendoglucanaseGH5MYP_3206endo-1,4-beta-mannosidaseendo-1,4-beta-mannosidaseGH5MYP_3455putativecarbohydrate-activeenzymeGH5MYP_3567endoglucanaseendoglucanaseGH8MYP_0051beta-glycosidase-likeproteinbeta-glycosidase-likeproteinGH8MYP_1583b-glycosidaseb-glycosidaseGH8MYP_1794chitinaseChitinaseGH8MYP_2076hypotheticalproteinGH8MYP_2112bifunctionalxylanase/esterasebifunctionalxylanase/esteraseGH8MYP_2156beta-glycosidase-l