文档介绍：福建医科大学
硕士学位论文
microRNA-192在胶质瘤中的表达及在胶质瘤细胞侵袭中作用的
研究
姓名:张涛
申请学位级别:硕士
专业:外科学(神经外科)
指导教师:王占祥
2011-05
microRNA-192 在胶质瘤中的表达及在胶质瘤细胞侵袭中作用的研究

中文摘要

目的:检测 miR-192 基因在人脑胶质瘤中的表达,及其表达情况与不同级别
胶质瘤的关系;观察 miR-192 对 U87 胶质瘤细胞迁移侵袭的影响,为进一步研究
脑胶质瘤细胞迁移和侵袭机制以及基因治疗提供理论基础。
方法:收集经病理确诊的 30 例不同恶性程度胶质瘤组织标本以及 10 例脑外
伤患者内减压术后的正常脑组织标本,提取总微小 RNA;应用实时定量 PCR 检
测 miR-192 基因在人脑胶质瘤组织和正常脑组织中的表达,并进一步比较
miR-192 表达情况与不同级别胶质瘤的相关性;应用基因转染技术上调 miR-192
水平;应用反义核酸技术抑制 miR-192 水平;real-time PCR 检测 miRNA 表达情
况;通过细胞划痕实验和细胞侵袭实验检测 miR-192 对 U87 胶质瘤细胞侵袭的
影响.
结果:1. miR-192 在胶质瘤中的表达水平较正常脑组织中高,且临床分级越
高,其表达水平越高。
2. 上调 miR-192 U87 细胞在细胞划痕实验和 transwell 小室细胞侵袭实验中
迁移侵袭的细胞数目升高;
3. 抑制 miR-192 U87 细胞在细胞划痕实验和 transwell 小室细胞侵袭实验中
迁移侵袭的细胞数目显著下降;
结论:1. miR-192 在胶质瘤组织中高表达,且表达水平呈现随胶质瘤级别增
高而增高趋势;
2. miR-192 具有促进胶质瘤细胞迁移侵袭的作用。
关键词:miR-192;胶质瘤;表达;迁移;侵袭

作者:张涛
指导教师:王占祥教授

4
The study on the expression level of miR-192 in gliomas and its effects on
glioma cell invasion
Abstract
Objective:To detect the expression level of miR-192 in human gliomas and its
correlation with its different clinical grades of gliomas ;To observe the influence of
miR-192 on glioma U87 cell’s invasion ,providing the theoretical basis for further
research of glioma cell’s migration and invasion mechanisms and gene therapy.
Methods:Tissues from 30 cases of gliomas confirmed by pathological types
and10 samples of normal human brain were collected from neurosurgical
pression operations on brain injury patients;Total miRNAs were
extracted;The expression level of miR-192 in human gliomas and normal brain
tissues was quantified by real-time polymerase chain reaction (Real-time PCR)
analysis respectively and the correlation of the miR-192's expression with glioma
grades was analyzed;The level of miR-192 was up-regulated by Gene carrying
technology;The level of miR-192 was suppressed by antisensenucleic acids
treatment;The level of miRNAs was detected by real-tim