蛋白质巯基检测要点.pdf

文档介绍：280 !""#ActaPharmaceuticaSinica2012, 47(3):280?290 !"#$%&'()*+,(-./01 !", #$% *, &'( *, )*+ ($%&'("!"), *+,-"./" (0123) 45678, *+200237) 23: 9-:;<=>?, ***@ABCDEFGH9-IJK, LMNOP4QRSTUVW, BXYZ[* \5]^_`abcdefghi, CDEFGjA9-JKHkblmnopqrst, ***@ABuvwxy zIop{|}~BD, !"#_`H$%c&'()*i+,opCDEFGjA9-JKH-"{- ./Q01, 2(G,FGJK$%345678jA+,CDE9"]^, 9-:;:TRS;g<=.b %>c456: 9-:;; CDEFG; 9-IJK; op; 345678 789:;:R916 <=>?@:A <AB;:0513-4870(2012)03-0280-11 Chemicalapproachesfortrappingproteinthiolsand theiroxidativemodification HUANGChu-sen, ZHUWei-ping *, XUYu-fang *, QIANXu-hong (Shanghai KeyLaboratoryof Chemical Biology, School ofPharmacy, East ChinaUniversityof ScienceandTechnology, Shanghai 200237, China) Abstract:Redoxsignaltransduction,especiallytheoxidativemodificationofproeinthiols,correlateswith esanexpandingresearch area. However,therewasraremethod for quick and specific detectionofproteinthiolsandtheiroxidativemodificationinlivingcells. Inthisarticle,wereviewthecurrent chemicalstrategiesforthe lookintothefutureofthedevelopmentoffluorescentprobesforproteinthiolsandtheirpotentialapplicationin theresearchofreactivecysteineproteomesandearlydetection ofredox-related diseases. Key words:redox;proteinthiol;oxidativemodification;detection;fluorescentprobe 9-:;?***@B./-"ABCQH56D EcuFG9HH3IJ, KL?***@HM/LNM/ OPQRSTUVHG9vI56, WXYvI 9Z(ROS) [\YvI]Z(RNS)cfghi,ROS ^_Xu./`yabcdIH?@, e9-***@f g, vI9HUhnijk"wxlPHm9-n opqg:2011-11-09; Jrqg:2011-12-15. GstX: Zu45Gv]^$%wx(973wx) yztX (2010CB126100); Zu{|}$%wx(863wx) y ztX(2011AA10A207); Zu~d"GsyztX (21076077). *O .\Tel/Fax:[phone], E-mail:******@ecust.;******@ecust. !, "e?#wxH$%[w&H'(cde))} *i, vI956*j9+6lk9-,-u<=> ?AH4Q.+./01"23c}~45H6` (*EGFRl7896`-)l:N (*;<=3-:l GEs>CD:-)l>?***@6 (*NF-κF,AP-1, OxyR) -CDEH<=>?ABC6vI9DE, 2FOPwx<=>? 9-:;<=>?LIJKlLMlNOSlPQR --OPMN4QRSTUVW, hXZ[*B\ SlT%BmlUVBXfWHXY]^_`abc u9-:;<=>?DEA, CQBOkZ[ X\CDEH9-:;]^6_H, ***@ABCDE `ab-: C