文档介绍：· 280 · 药学学报 Acta Pharmaceutica Sinica 2012, 47 (3): 280−290
蛋白质巯基及其氧化性修饰的化学检测方法
黄楚森, 朱维平*, 徐玉芳*, 钱旭红
(华东理工大学药学院, 上海市化学生物学 (芳香杂环) 重点实验室, 上海 200237)
摘要: 氧化还原信号转导, 尤其是蛋白质巯基的氧化性修饰, 与人类许多重要疾病关系密切, 是目前国际上
热点研究领域之一。然而长期以来, 蛋白质巯基及其氧化修饰的专一、快速检测手段缺乏, 尤其是在活细胞内
源性检测方面几乎是空白, 限制了该领域的发展。本文就近年来用于检测蛋白质巯基及其氧化修饰的化学方法
作简要综述, 并就基于巯基修饰发展荧光分子探针及其用于蛋白质组学研究, 氧化还原相关疾病早期诊断作一
展望。
关键词: 氧化还原; 蛋白质巯基; 氧化性修饰; 检测; 荧光分子探针
中图分类号: R916 文献标识码: A 文章编号: 0513-4870 (2012) 03-0280-11
Chemical approaches for trapping protein thiols and
their oxidative modification
HUANG Chu-sen, ZHU Wei-ping*, XU Yu-fang*, QIAN Xu-hong
(Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology,
Shanghai 200237, China)
Abstract: Redox signal transduction, especially the oxidative modification of proein thiols, correlates with
many diseases and becomes an expanding research area. However, there was rare method for quick and specific
detection of protein thiols and their oxidative modification in living cells. In this article, we review the current
chemical strategies for the detection and quantification of protein thiols and related cysteine oxidation. We also
look into the future of the development of fluorescent probes for protein thiols and their potential application in
the research of reactive cysteine proteomes and early detection of redox-related diseases.
Key words: redox; protein thiol; oxidative modification; detection; fluorescent prob