文档介绍：Industrial Microbiology and Fermentation Technology
Textbook: Biology of anisms
Seventh Edition
By Thomas D. Brock et al. (1996)
Website: /~brock
Lecturer: CHEN Guo-Qiang (陈国强)
******@public3..cn
Tel: 62783844, Room 102, School of Life Sciences
Industrial Microbiology and Fermentation Technology
Industrial Microbiology is the discipline that uses anisms, usually grown on a large scale, to produce mercial products or carry out important chemical transformations.
Fermentation Technology is the technology to grow cells in a large scale with high efficiency, it also includes product recovery processes.
Microbial products: microbial cells, enzymes,
pharmaceutical products
Specialty Chemicals and food additives
Flowsheet for developing an industrial microbial fermentation process
Strain selection
Laboratory process development
Pilot Scale up
Industrial Scale up
Downstream process development
Product packaging techniques
mercial consideration
Examples
Strain Selection
Purchase from Culture Collections
Screening of nature circumstances
ic engineering
Mutations
Cell biology techniques
International Culture Collections
Domestically, strains can be purchased from: or
China General Microbiological Culture Collection Center
中国微生物菌种保藏管理委员会普通微生物中心,北京中关村
Screening of nature circumstances
ic Engineering
Incorporation into
artificial plasmids of
genes from a wide
variety of sources has
made possible the
transfer of ic
material across
virtually any species
barrier
Various high value
added products have
been produced from
the ic engineering
methods
Mutation
Via chemical
or physical,
and biological
means
Cell biology techniques
Protoplast fusion (promote high frequencies of ic binants)
removing the cell wall with lytic enzymes in the presence of osmotic stabilizers.
In the presence of fusogenic agent such as polyethylene glycol (PEG), protoplasts are induced to fuse and form transient hybrids or diploids.
Regeneration of viable cells from the fused pr