文档介绍：e Each of You to My Molecular Biology Class
Molecular Biology of the Gene, 5/E --- Watson et al. (2004)
Part I: Chemistry and ics
Part II: Maintenance of the Genome
Part III: Expression of the Genome
Part IV: Regulation
Part V: Methods
3/15/05
Part II: Maintenance of the Genome
Dedicated to the structure of DNA and the processes that propagate, maintain and alter it from one cell generation to the next
Ch 6: The structures of DNA and RNA
Ch 7: Chromosomes, chromatins and the nucleosome
Ch 8: The replication of DNA
Ch 9: The mutability and repair of DNA
Ch 10: Homologous bination at the molecular level
Ch 11: Site-specific bination and transposition of DNA
3/15/05
CHAPTER 8: The replication of DNA
Molecular Biology Course
Teaching Arrangement
Watch animation-Understand replication
Go through structural tutorial-Experience the BEAUTY of the nature
prehensive understanding of Chapter 8
Summary and highlight Key points
CHAPTER 8 The replication of DNA
The Chemistry of DNA Synthesis
The Mechanism of DNA Polymerase
The Replication Fork
The Specialization of DNA Polymerases
DNA Synthesis at the Replication Fork
Initiation of DNA Replication
Binding and Unwinding
Finishing Replication
General
Detailed
CHAPTER 8 The replication of DNA
The first part describes the basic chemistry of DNA synthesis and the function of the DNA polymerase
CHAPTER 8 The replication of DNA
The Chemistry of DNA
CHAPTER 8 The replication of DNA
DNA synthesis requires deoxynucleoside triphosphates and a primer:template junction
DNA is synthesized by extending the 3’ end of the primer
Hydrolysis of pyrophosphate (PPi) is the driving force for DNA synthesis
3/18/05
Figure 8-3 Substrate required for DNA synthesis