文档介绍：Getting More From Your Malaria Molecular Data
on the Web:
Chuong Huynh
National Center for
Biotechnology Information,
National Library of Medicine,
National Institute of Health
A Survey of PlasmoDB, MGP,
NCBI Resources
Homology
.. Is the central concept of all biology. Whenever we say that a mammalian hormone is the ‘same’ hormone as a fish hormone, that a human gene sequence is the ‘same’ as a sequence in a chimp or a mouse, that a HOX gene is the ‘same’ in a mouse, a fruit fly, a frog, a human – even when we argue that discoveries about a worm, a fruit fly, a frog, a mouse, or a chimp have relevance to the human condition –we have made a bold and direct statement about homology. The aggressive confidence of a modern biomedical science implies that we know what we are talking about.”- David B. Wake
The Big Picture Concept
Comparative analysis in biology -. Leishmania and Plasmodium
Genotype to Phenotype
> Protein sequence
MKIVYWSGTGNTEKMAELIAKGIIESGKDVNTINVSDVNI
DELLNEDILILGCSAMGDEVLEESEFEPFIEEISTKISGK
KVALFGSYGWGDGKWMRDFEERMNGYGCVVVETPLIVQNE
PDEAEQDCIEFGKKIANI
> DNA sequence
GGCAACAC
TGAGAAAATGGCAGAGCTCATCGCTAAAGGTATCATCGAA
ATCAACGTGTCTGACGTTA
TGGG
ATGGGCGATGAAGTTCTCGAGGAAAGCGAA
AAAATCTCTG
GTAAGAAGGTTGCGCTGTTCGGTTCTTACGGTTGGGGCGA
CGGTAAGTGGATGCGTGACTTCGAAGAACGTATGAACGGC
GCTGATCGTTCAGA
GGACGAAGCTGAGCAGGACTGCATCGAATTTGG
TAAGAAGATCGCGAACATCTAGTAGA
The Big Picture Concept
Colon cancer gene sequence
3000 Myr
1000 Myr
500 Myr
Human
Fly
Worm
Yeast
Bacteria
Mouse
Human 638 RHACVEVQDEIAFIPNDVYFEKDKQMFHIITGPNMGGKSTYIRQTGVIVLMAQIGCFVPC 697
Yeast 657 RHPVLEMQDDISFISNDVTLESGKGDFLIITGPNMGGKSTYIRQVGVISLMAQIGCFVPC 716
584 RHPVVEQVLNEPFIANPLNLSPQRR-MLIITGPNMGGKSTYMRQTALIALMAYIGSYVPA 642
Homology implies mon ancestry which
may be inferred from observations
of sequence similarity
The Big Plan
1: Human Genome Resources
Information on:
* Genes - LocusLink & Unigene
* Genomes Maps (MapViewer)
* Sequence Data (contig, unigene,
dbEST, Sequence View