文档介绍：Sepharose and Sepharose CL INSTRUCTIONS
Sepharose™ is a bead-formed agarose-based gel filtration matrix. Sepharose is
available with 3 different agarose contents; 2, 4, and 6%, designated Sepharose
2B, Sepharose 4B and Sepharose 6B respectively.
Sepharose CL gels are cross-linked derivatives of Sepharose 2B, Sepharose 4B
and Sepharose 6B. The cross-linked form of Sepharose is chemically and physi-
cally more resistant than Sepharose itself, offering the same selectivity with bet-
ter flow characteristics. Cross-linked Sepharose gels are resistant to organic sol-
vents and are thus the choice for separations in organic solvents.
Both Sepharose and Sepharose CL have broad fractionation ranges which
makes them suitable for characterizing or cleaning-up samples containing com-
ponents of diverse molecular weight.
71-7098-00
Edition AC
Table 1. Gel characteristics
Sepharose 2B 4B 6B CL-2B CL-4B CL-6B
% agarose 2 4 6 2 4 6
Optimal MW 70x103-40x106 70x103-20x106 10x103-4x106 70x103-40x106 70x103-20x106 10x103-4x106
separation range
(globular proteins)
Bead size range (µm) 60-200